Probing the Aggregation and Immune Response of Human Islet Amyloid Polypeptides with Ligand-Stabilized Gold Nanoparticles
Probing the Aggregation and Immune Response of Human Islet Amyloid Polypeptides with Ligand-Stabilized Gold Nanoparticles
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配体稳定的金纳米粒子对人胰岛淀粉样多肽聚集和免疫应答的研究
DOI：
10.1021/acsami.8b19506
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发表时间：
2019-03-20
影响因子：
9.5
通讯作者：
Chen, Pengyu
中科院分区：
文献类型：
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作者：
Javed, Ibrahim;He, Jiacheng;Chen, Pengyu
纳米材料的使用最近已成为对抗与一系列代谢和脑疾病相关的蛋白质淀粉样变性的新兴策略。为了促进这一领域的研究，我们首先演示了使用高光谱成像 (HSI) 和 COMSOL 模拟来报告人类胰岛淀粉样多肽 (IAPP) 的聚集（2 型糖尿病的标志），以及肽和接枝柠檬酸盐和聚乙二醇的金纳米颗粒 (AuNP) 之间的物理相互作用。 （PEG(400) 和 PEG(3000)）。我们发现 IAPP 聚集增加与 AuNP 等离子体共振引起的光谱红移减少之间存在明显的反相关性。此外，暴露于 IAPP 和 AuNP 的 Jurkat 细胞的特征是通过局部表面等离子共振 (LSPR) 免疫测定定量其细胞因子分泌，其中毒性最强的 IAPP 寡聚物出现峰值反应，并且大多数被柠檬酸盐包被的 AuNP 抑制。这项研究证明了使用 HSI 和 LSPR 作为两个新平台的潜力，可以轻松检查与淀粉样蛋白疾病相关的蛋白质聚集及其诱导的免疫反应。
The use of nanomaterials has recently become an emerging strategy against protein amyloidosis associated with a range of metabolic and brain diseases. To facilitate research in this area, here we first demonstrated the use of hyperspectral imaging (HSI) and COMSOL simulations for reporting the aggregation of human islet amyloid polypeptides (IAPPs), a hallmark of type 2 diabetes, as well as the physical interactions between the peptide and gold nanoparticles (AuNPs) grafted with citrate and poly(ethylene glycol) (PEG(400) and PEG(3000)). We found a distinct anticorrelation between increased IAPP aggregation and decreased spectral red shifts incurred in the AuNP plasmonic resonance. Moreover, Jurkat cells exposed to IAPP and AuNPs were characterized by quantifying their cytokine secretions with a localized surface plasmon resonance (LSPR) immunoassay, where a peak response was registered for the most toxic IAPP oligomers and most suppressed by citrate-coated AuNPs. This study demonstrated the potential of using HSI and LSPR as two new platforms for the facile examination of protein aggregation and their induced immune response associated with amyloid diseases.