TLR6: A novel member of an expanding Toll-like receptor family
TLR6: A novel member of an expanding Toll-like receptor family
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DOI：
10.1016/s0378-1119(99)00098-0
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发表时间：
1999-04-29
期刊：
影响因子：
3.5
通讯作者：
Akira, S
中科院分区：
文献类型：
--
作者：
Takeuchi, O;Kawai, T;Akira, S
果蝇Toll蛋白可以激活成虫的先天免疫系统，调节发育中胚胎的背腹模式。最近，已发现果蝇Toll的五个人类同源物，它们被命名为Toll样受体(Toll-like Receptor，TLRs)，并被证明在先天性免疫反应中发挥作用。我们报道了Toll样受体家族的一个新成员Toll样受体6(TLR6)的分子克隆和鉴定。人和小鼠TLR6是I型跨膜受体，既含有胞外富含亮氨酸重复序列(LRR)结构域，又含有胞内Toll/IL-1受体(IL-1R)样区。人TLR6(HTLR6)的氨基酸序列与hTLR1最为相似，有69%的同源性。RT-PCR分析表明，小鼠TLR6主要在脾、胸腺、卵巢和肺中表达。与其他TLR家族成员一样，结构活性TLR6可同时激活核因子-kappaB和c-jun氨基末端激酶(JNK)。TLR6基因和TLR1基因被定位到小鼠5号染色体的近端区域，彼此相距不到1.7厘米。这些结果表明，TLR6是一个正在扩大的TLR家族的新成员。(C)1999 Elsevier Science B.V.保留所有权利。
Drosophila Toll protein is shown to activate the innate immune system in adult and regulate the dorsoventral patterning in the developing embryo. Recently, five human homologs of Drosophila Toll, designated as Toll-like receptors (TLRs), have been identified and shown to play a role in the innate immune response. We report here the molecular cloning and characterization of a new member of Toll-like receptor family, Toll-like receptor 6 (TLR6). Human and murine TLR6 are type-I transmembrane receptors that contain both an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic Toll/IL-1 receptor (IL-1R)-like region. The amino acid sequence of human TLR6 (hTLR6) is most similar to that of hTLR1 with 69% identity. RT-PCR analysis revealed that murine TLR6 is expressed predominantly in spleen, thymus, ovary and lung. Like other TLR family members, constitutively active TLR6 activates both NF-kappa B and c-Jun N-terminal kinase (JNK). The TLR6 gene, as well as the TLR1 gene, mapped to the proximal region of murine chromosome 5 within 1.7 cM of each other. These results suggest that TLR6 is a novel member of an expanding TLR family. (C) 1999 Elsevier Science B.V. All rights reserved.