Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: Fabrication, characterization, and enzymatic activity
Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: Fabrication, characterization, and enzymatic activity
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DOI：
10.1021/ac981300q
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发表时间：
1999-08-01
影响因子：
7.4
通讯作者：
Pishko, MV
中科院分区：
文献类型：
--
作者：
Franchina, JG;Lackowski, WM;Pishko, MV
在这篇文章中，我们发现超支化聚合物可以用作静电捕获酶的基质，特别是胺基官能化葡萄糖氧化酶(Gox(+))和辣根过氧化物酶，以及聚酰胺胺树枝状大分子修饰的辣根过氧化物酶，可逆地吸附到聚阴离子超支化聚丙烯酸钠(PAA(-))膜上，这些膜的厚度约为几百埃。包埋在PAA(-)膜中的Gox(+)的量取决于膜制备的性质，但通常约为0.06个单位/厘米(2)。包埋的Gox(+)保持其活性的程度取决于膜的历史，但对于不暴露于葡萄糖并在4℃下储存的PAA(-)/Gox(+)复合材料，原始活性保持长达68天或更长时间。
In this paper we show that hyperbranched polymers can be used as a host matrix for electrostatic entrapment of enzymes, Specifically, amine-functionalized glucose oxidase (GOx(+)) and horseradish peroxidase, as well as poly(amidoamine) dendrimer-modified horseradish peroxidase, reversibly sorb into polyanionic, hyperbranched poly(sodium acrylate) (PAA(-)) films that are on the order of a few hundred angstroms thick. The quantity of GOx(+) entrapped within the PAA(-) films depends on the nature of film preparation but is typically on the order of 0.06 unit/cm(2). The extent to which entrapped GOx(+) retains its activity depends on the film history, but for PAA(-)/GOx(+) composites not exposed to glucose and stored at 4 degrees C, the original activity is retained for up to 68 days and perhaps longer.