Dynamic intracellular localization of Dazl protein during Xenopus germline development
Dynamic intracellular localization of Dazl protein during Xenopus germline development
复制标题
非洲爪蟾种系发育过程中 Dazl 蛋白的动态细胞内定位
DOI：
10.1007/s00418-015-1323-5
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发表时间：
2015
影响因子：
2.3
通讯作者：
Haru Tada and Hidefumi Orii
中科院分区：
文献类型：
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作者：
長谷川 敦史;清水 律子;金子 寛;山本 雅之;長澤伸樹;長澤伸樹;長澤 伸樹;長澤 伸樹;前田健一，近藤明彦;近藤 明彦;近藤 明彦;近藤明彦;近藤 明彦;Haru Tada and Hidefumi Orii
非洲爪哇编码一种RNA结合蛋白，已被鉴定为种质的一种成分，并参与原始生殖细胞的迁移和分化。在这里，我们研究了DAZL在非洲爪哇整个生命周期中在生殖细胞中的细胞内定位。在胚胎发生的早期，DAZL最初在种质中被检测到，然后被转移到核周区域。然后，在PGCs的胞核内检测到它。当性腺开始分化时，DAZL仅见于PGCs的细胞质中。DAZL在初级卵原细胞和精原细胞的胞核和胞浆中均有分布，但仅在次级卵原细胞和精原细胞的胞浆中分布。在精母细胞中，DAZL分布于细胞质中，在减数分裂过程中定位于纺锤体和细胞质。然后，在圆形精子细胞的细胞核内检测到斑点。DAZL在细胞内的动态定位表明，DAZL是一种多功能蛋白质，调节着Xenopusgermline发育所需的RNA代谢。
Xenopus dazlencoding an RNA-binding protein has been identified as a component of the germ plasm and is involved in the migration and differentiation of the primordial germ cells (PGCs). Here, we investigated the intracellular localization of Dazl in germline cells throughout the lifetime ofXenopus. In early embryogenesis, Dazl was detected initially in the germ plasm and then translocated to a perinuclear region. Then, it was detected within the nucleus in PGCs. Dazl was observed only in the cytoplasm in PGCs when sex differentiation began in the gonads. Dazl was distributed in both the nucleus and cytoplasm of the primary oogonium and spermatogonium, but only in the cytoplasm of the secondary oogonium and spermatogonium. In spermatocytes, Dazl was distributed throughout cytoplasm and localized at the spindles and cytoplasm during meiosis. Then, it was detected as speckles in the nucleus in the round spermatid. The dynamic intracellular localization suggests that Dazl is a multifunctional protein regulating RNA metabolism required forXenopusgermline development.