High G+C Content of Herpes Simplex Virus DNA: Proposed Role in Protection Against Retrotransposon Insertion.
High G+C Content of Herpes Simplex Virus DNA: Proposed Role in Protection Against Retrotransposon Insertion.
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DOI：
10.2174/1874091x00701010033
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发表时间：
2007-12-04
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影响因子：
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通讯作者：
Brown, Jay C
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文献类型：
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作者：
Brown, Jay C
单纯疱疹病毒dsDNA基因组具有异常高的G+C核苷酸含量。例如，HSV-1和HSV-2的GC含量分别为68%和70%，而宿主(人类)基因组的GC含量为41%。为了确定GC含量如何随着基因组位置的变化而变化，我们分别测量了HSV-1 DNA编码区和基因间隔区的GC含量。结果表明，75个基因构成了一个均匀的群体，平均GC含量为66.9+/-4.1%。相反，在两个不重叠的群体中发现了基因间隔区，其中一个的平均GC含量(69.3+/-4.6%n=32)与编码区相近，而另一个的GC含量较低(56.0+/-4.9 n=30)。与基因组的其他区域相比，GC含量降低的基因间区富含局部GC Minimum、CACACA序列和逆转座子事件的主要靶序列(TTAAAA)。这些结果被解释为，高GC含量是HSV-1保护其基因免受神经系统细胞分化过程中活跃的移动遗传元件入侵的方式的一部分。
The herpes simplex virus dsDNA genome is distinguished by an unusually high G+C nucleotide content. HSV-1 and HSV-2, for instance, have GC contents of 68% and 70% respectively, while that of the host (human) genome is 41%. To determine how GC content varies with genome location, GC content was measured separately in coding and intergenic regions of HSV-1 DNA. The results showed that the 75 genes constitute a uniform population with a mean GC content of 66.9 +/- 4.1%. In contrast, intergenic regions were found in two non-overlapping populations, one with a mean GC content (69.3 +/- 4.6% n=32) similar to the coding regions and another where the GC content is lower (56.0 +/- 4.9 n=30). Compared to other regions of the genome, intergenic regions with reduced GC content were found to be enriched in local GC minima, CACACA sequences and a primary target sequence (TTAAAA) for retrotransposition events. The results are interpreted to suggest that a high GC content is part of the way HSV-1 protects its genes from invasion by mobile genetic elements active during cell differentiation in the nervous system.