The Molecular Anatomy of Mouse Skin during Hair Growth and Rest
The Molecular Anatomy of Mouse Skin during Hair Growth and Rest
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DOI：
10.1016/j.stem.2020.01.012
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发表时间：
2020-03-05
期刊：
影响因子：
23.9
通讯作者：
Kasper, Maria
中科院分区：
文献类型：
--
作者：
Joost, Simon;Annusver, Karl;Kasper, Maria
皮肤内稳态是由几十种细胞类型精心安排的，它们共同指导干细胞更新、谱系承诺和分化。在这里，我们使用单细胞RNA测序和单分子RNA FISH来提供全层皮肤的系统分子图谱，确定基因表达谱和空间位置，定义毛发生长和休息期间56种细胞类型和状态。这些发现揭示了外根鞘（ORS）和内毛囊层是如何协调头发生长的。我们发现ORS是由两种混合但转录不同的细胞类型组成的，它们与基质细胞类型的相互作用能力不同。内层细胞从转录未承诺的祖细胞分支，每个谱系分化经过一个中间状态。我们还提供了一个在线工具来探索这个全面的皮肤细胞图谱，包括上皮细胞和基质细胞，如成纤维细胞、血管细胞和免疫细胞，以促进皮肤生物学的进一步发现。
Skin homeostasis is orchestrated by dozens of cell types that together direct stem cell renewal, lineage commitment, and differentiation. Here, we use single-cell RNA sequencing and single-molecule RNA FISH to provide a systematic molecular atlas of full-thickness skin, determining gene expression profiles and spatial locations that define 56 cell types and states during hair growth and rest. These findings reveal how the outer root sheath (ORS) and inner hair follicle layers coordinate hair production. We found that the ORS is composed of two intermingling but transcriptionally distinct cell types with differing capacities for interactions with stromal cell types. Inner layer cells branch from transcriptionally uncommitted progenitors, and each lineage differentiation passes through an intermediate state. We also provide an online tool to explore this comprehensive skin cell atlas, including epithelial and stromal cells such as fibroblasts, vascular, and immune cells, to spur further discoveries in skin biology.