Aqueous humor and plasma concentrations of a compounded 0.2% solution of terbinafine following topical ocular administration to normal equine eyes
Aqueous humor and plasma concentrations of a compounded 0.2% solution of terbinafine following topical ocular administration to normal equine eyes
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DOI：
10.1111/j.1463-5224.2010.00841.x
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Gilger, Brian
中科院分区：
文献类型：
--
作者：
Clode, Alison;Davis, Jennifer;Gilger, Brian
目的测定正常马眼重复局部给药后复方0.2%特比萘芬溶液的经角膜渗透和全身吸收。样本群体6匹眼科检查正常的健康成年马。操作方法每4小时每匹马一只眼接受0.2mL复方0.2%特比萘芬溶液，共7次。在施用最终剂量后的 1 小时内，获得多个外周血样本，并在一小时结束时收集单个房水 (AH) 样本。使用高压液相色谱 (HPLC) 测定特比萘芬的 AH 和血浆浓度。在 14 天的时间段内通过 HPLC 分析评估制剂的稳定性。结果在任何时间点的任何马的 AH 或血浆中均未检测到特比萘芬。在任何时间点，任何马都没有发现眼部刺激或全身毒性的迹象。该溶液在 14 天内保持稳定。结论 0.2% 特比萘芬复方溶液局部眼部给药至正常马眼后不会导致可检测到的 AH 或血浆水平，表明其在马眼科治疗深部角膜或眼内真菌感染中的应用可能受到限制。
ObjectiveTo determine the transcorneal penetration and systemic absorption of a compounded 0.2% terbinafine solution following repeated topical administration to normal equine eyes.Sample populationSix healthy adult horses with normal ocular examinations.ProceduresOne eye of each horse received 0.2 mL of a compounded 0.2% terbinafine solution every 4 h for seven doses. During the 1 h following administration of the final dose, multiple peripheral blood samples were obtained, and a single aqueous humor (AH) sample was collected at the end of the hour. AH and plasma concentrations of terbinafine were determined using high pressure liquid chromatography (HPLC). Stability of the formulation was assessed with HPLC analysis over a 14-day time period.ResultsTerbinafine was not detected in the AH or plasma of any horse at any time point. No signs of ocular irritation or systemic toxicity were noted in any horse at any time point. The solution was stable over 14 days.ConclusionTopical ocular administration of compounded 0.2% terbinafine solution does not result in detectable AH or plasma levels following administration to normal equine eyes, suggesting its use for deep corneal or intraocular fungal infections in equine ophthalmology may be limited.