Singapore Ocular Tuberculosis Immunity Study (SPOTIS): Role of T-lymphocyte Profiling in Patients with Presumed Ocular Tuberculosis
Singapore Ocular Tuberculosis Immunity Study (SPOTIS): Role of T-lymphocyte Profiling in Patients with Presumed Ocular Tuberculosis
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新加坡眼结核免疫研究 (SPOTIS)：T 淋巴细胞分析在疑似眼结核患者中的作用
DOI：
10.1080/09273948.2020.1767791
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发表时间：
2020
影响因子：
3.3
通讯作者：
Siak Jay
中科院分区：
文献类型：
--
作者：
Hutchinson Paul E.;Kee Ae Ra;Agrawal Rupesh;Yawata Nobuyo;Tumulak Mayjane Jg;Connolly John E.;Chee Soon-Phaik;Siak Jay
目的：通过前瞻性临床研究，评价外周血中CD4+T细胞亚群在眼结核病(TB)中的应用价值。结果：治疗应答者与无应答者PPD或ESAT-6+、CFP-10特异性CD4+T细胞比例无显著差异，但治疗应答者外周血中CD38+、CD38+、HLADR+T细胞比例明显高于无应答者(P=0.0357)。结论：本研究是首次探讨眼结核病患者外周血中MTB特异性T细胞活化标志物的表达情况。需要进行进一步的研究，以评估CD4++T细胞表型作为眼部结核病生物标志物的实用性。
Objective: A prospective clinical study to assess the utility of CD4 + T cell lymphocyte profiling from peripheral blood in patients with ocular tuberculosis (TB).Methods: Thirty-six Asian patients with presumed diagnosis of ocular TB were recruited for T-lymphocyte profiling. MTB antigen specific CD4 assay was set up, and flow cytometric data were analyzed using FlowJo software.Results: There was no significant difference between treatment responders and non-responders for the proportion of CD4 + T cells specific for PPD or ESAT-6+ CFP-10, but treatment responders did have significantly higher frequency of CD38+ (p= .0357) and CD38+ HLA-DR+ (p= .0357) on the PPD-specific CD4 + T cells.Conclusion: This study is one of the first of its kind to look into MTB specific T cell activation marker profiling of peripheral blood in patients with ocular TB. Further studies need to be undertaken to assess the utility of CD4 + T cell phenotypes as a biomarker for ocular TB.