RADIOIMMUNOASSAY OF CHROMOGRANIN-A IN PLASMA AS A MEASURE OF EXOCYTOTIC SYMPATHOADRENAL ACTIVITY IN NORMAL SUBJECTS AND PATIENTS WITH PHEOCHROMOCYTOMA
RADIOIMMUNOASSAY OF CHROMOGRANIN-A IN PLASMA AS A MEASURE OF EXOCYTOTIC SYMPATHOADRENAL ACTIVITY IN NORMAL SUBJECTS AND PATIENTS WITH PHEOCHROMOCYTOMA
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DOI：
10.1056/nejm198409203111204
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发表时间：
1984-01-01
影响因子：
158.5
通讯作者：
BERNSTEIN, KN
中科院分区：
文献类型：
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作者：
OCONNOR, DT;BERNSTEIN, KN
嗜铬粒蛋白A是一种主要的可溶性蛋白，与儿茶酚胺一起由肾上腺髓质和交感神经的囊泡储存和分泌。通过对嗜铬细胞瘤小泡中纯化的嗜铬粒蛋白A进行放射免疫测定，探讨了嗜铬粒蛋白A作为外泌性交感肾上腺活性探针的可能性。18例正常卧位男性血清嗜铬粒蛋白A浓度为129。12 ng / ml。其浓度随交感肾上腺活性的生理、药理学和病理变化而变化。它站着时上升，躺着时下降，虽然它不受短暂的动态运动的干扰。它在短暂的血管舒张时升高，在酪胺刺激非胞外性儿茶酚胺释放和神经节阻滞时下降。11例嗜铬细胞瘤患者血浆嗜铬粒蛋白A浓度升高(1614 +-。408 ng / ml)。平均血浆半衰期为18.4 min。正常人静息和激活的交感肾上腺张力，以及嗜铬细胞瘤分泌的儿茶酚胺，在机制上至少部分是胞外分泌的。嗜铬粒蛋白A可能是胞外性交感肾上腺活性的有用探针，也是嗜铬细胞瘤的有用诊断标志物。
Chromogranin A is the major soluble protein stored and secreted by exocytosis, along with catecholamines, from vesicles in the adrenal medulla and sympathetic nerves. The possible use of chromogranin A as a probe of exocytotic sympathoadrenal activity was investigated by developing a radioimmunoassay for chromogranin A purified from pheochromocytoma vesicles. In 18 normal recumbent men chromogranin A was present in plasma at a concentration of 129 .+-. 12 ng/ml. The concentration varied with physiologic, pharmacologic, and pathologic changes in sympathoadrenal activity. It rose with standing and fell with recumbency, though it was not perturbed by brief dynamic exercise. It rose during brief vasodilation and fell during stimulation of nonexocytotic catecholamine release by tyramine and during ganglionic blockade. The plasma concentration of chromogranin A was elevated in 11 patients with pheochromocytoma (1614 .+-. 408 ng/ml). The mean plasma half-life was 18.4 min. Both resting and activated sympathoadrenal tone in normal persons, as well as catecholamine secretion by pheochromocytoma, are at least in part exocytotic in mechanism. Chromogranin A may be a useful probe of exocytotic sympathoadrenal activity as well as a useful diagnostic marker for pheochromocytoma.