Hybrid plasmonic magnetic nanoparticles as molecular specific agents for MRI/optical imaging and photothermal therapy of cancer cells
Hybrid plasmonic magnetic nanoparticles as molecular specific agents for MRI/optical imaging and photothermal therapy of cancer cells
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DOI：
10.1088/0957-4484/18/32/325101
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发表时间：
2007-08-15
期刊：
影响因子：
3.5
通讯作者：
Sokolov, Konstantin
中科院分区：
文献类型：
--
作者：
Larson, Timothy A.;Bankson, James;Sokolov, Konstantin
由等离子体层和氧化铁部分组成的纳米颗粒可以为未来医学中的多模态成像和治疗方法的开发提供有前途的平台。然而，这一平台的可行性还有待充分探讨。在这项研究中，我们证明了使用金包被的氧化铁混合纳米粒子的组合分子特异性MRI/光学成像和光热治疗的癌细胞。金层表现出表面等离子体共振，其由于可见光区域中的光散射而提供光学对比度，并且还呈现用于缀合靶向部分的方便表面，而氧化铁核给出强的T-2（自旋-自旋弛豫时间）对比度。等离子体金层的强光学吸收也使这些纳米颗粒成为光热治疗的有前途的试剂。我们合成了特异性靶向表皮生长因子受体（EGFR）的混合纳米颗粒，EGFR是许多上皮癌的常见生物标志物。我们在MDA-MB-468乳腺癌细胞中展示了分子特异性MRI和光学成像。此外，我们发现，受体介导的抗EGFR混合纳米颗粒的聚集允许使用纳秒脉冲激光在700 nm波长下选择性破坏高度增殖的癌细胞，这与分离的混合纳米颗粒在532 nm处的峰值吸光度相比有显著偏移。
Nanoparticles which consist of a plasmonic layer and an iron oxide moiety could provide a promising platform for development of multimodal imaging and therapy approaches in future medicine. However, the feasibility of this platform has yet to be fully explored. In this study we demonstrated the use of gold-coated iron oxide hybrid nanoparticles for combined molecular specific MRI/optical imaging and photothermal therapy of cancer cells. The gold layer exhibits a surface plasmon resonance that provides optical contrast due to light scattering in the visible region and also presents a convenient surface for conjugating targeting moieties, while the iron oxide cores give strong T-2 (spin-spin relaxation time) contrast. The strong optical absorption of the plasmonic gold layer also makes these nanoparticles a promising agent for photothermal therapy. We synthesized hybrid nanoparticles which specifically target epidermal growth factor receptor (EGFR), a common biomarker for many epithelial cancers. We demonstrated molecular specific MRI and optical imaging in MDA-MB-468 breast cancer cells. Furthermore, we showed that receptor-mediated aggregation of anti-EGFR hybrid nanoparticles allows selective destruction of highly proliferative cancer cells using a nanosecond pulsed laser at 700 nm wavelength, a significant shift from the peak absorbance of isolated hybrid nanoparticles at 532 nm.