Regulation of Caenorhabditis elegans Male Mate Searching Behavior by the Nuclear Receptor DAF-12
Regulation of Caenorhabditis elegans Male Mate Searching Behavior by the Nuclear Receptor DAF-12
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DOI：
10.1534/genetics.108.093773
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发表时间：
2008-12-01
期刊：
影响因子：
3.3
通讯作者：
Emmons, Scott W.
中科院分区：
文献类型：
--
作者：
Kleemann, Gunnar;Jia, Lingyun;Emmons, Scott W.
动物行为与生殖状态的协调通常是通过性腺分泌激素来实现的。在秀丽隐杆线虫中，成年雄性探索它们的环境以寻找配偶。寻找配偶的过程受配偶的存在、营养状况和性腺信号的影响。在这里，我们表明，性腺信号的行为通过核受体ESTA-12，一种蛋白质，已知调节几个线虫的生活史特征。β-12具有激活和组织功能来刺激探索行为Mid作用于性腺信号的下游，在性腺之外。β-12作用于来自交配伴侣的感觉输入和指示营养状态的生理信号的上游。在生殖系切除的动物中，交配搜索被拯救，但如果生殖系和体细胞性腺都被切除，则由21 -12配体的前体dafachronic acid（DA）。结果，被解释为表明，生殖系产生的DA前体被转换为DA外的生殖系，可能在体细胞性腺。正如它在其他途径中发挥作用一样，在调节雄性择偶行为中，α-12起作用。在有利于繁殖（寻找配偶）和生存（依靠食物）的选择之间的选择点。
Coordination of animal behavior with reproductive status is often achieved through elaboration Of hormones by the gonad. In the nematode Caenorhabditis elegans, adult males explore their environment to locate mates. Mate searching is regulated by presence of mates, nutritional status, and a signal from the gonad. Here we show that the gonadal signal acts via the nuclear receptor DAF-12, a protein known to regulate several G elegans life-history traits. DAF-12 has both activational and organizational functions to stimulate exploratory behavior Mid acts downstream Of the gonadal signal, outside of the gonad. DAF-12 acts upstream of sensory input from mating partners and physiological signals indicating nutritional status. Mate searching was rescued in germ-line ablated animals, but not if both germ line and somatic gonad were ablated, by 21 precursor of the DAF-12 ligand, dafachronic acid (DA). The results, are interpreted to suggest that the germ line produces a DA precursor that is converted to DA outside of the germ line, possibly in the somatic gonad. As it does in other pathways in Which it functions, in regulation of male mate searching behavior DAF-12 act,; at. a choice point between alternatives favoring reproduction (mate searching) vs. survival (remaining on food).