Treatment of cirrhotic rats with epidermal growth factor and insulin accelerates liver DNA synthesis after partial hepatectomy
Treatment of cirrhotic rats with epidermal growth factor and insulin accelerates liver DNA synthesis after partial hepatectomy
复制标题
表皮生长因子和胰岛素治疗肝硬化大鼠部分肝切除后加速肝脏 DNA 合成
DOI：
--
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发表时间：
1998
影响因子：
4.1
通讯作者：
S. Raper
中科院分区：
文献类型：
--
作者：
M. Hashimoto;P. Kothary;F. Eckhauser;S. Raper
Prevention of postoperative hepatic failure is important after hepatic resection. In patients with cirrhosis, impaired liver function and regenerative capacity after major hepatic resection are associated with increased morbidity and mortality. In this study, a combination of epidermal growth factor (EGF) and insulin were used as hepatotrophic factors in an attempt to stimulate DNA synthesis after 70% hepatectomy (HTX). Regenerative capacity was evaluated in normal and cirrhotic rat liver by measuring DNA synthesis in vivo. Micronodular liver cirrhosis was established by the simultaneous oral administration of CCl4 and phenobarbital. Epidermal growth factor plus insulin was injected subcutaneously immediately after and 12 h after HTX or sham operation was performed. Rats were killed 24 h after the operation and liver regeneration was estimated by [3H]‐thymidine incorporation into DNA as well as an autoradiographic nuclear labelling index. Hepatectomy increased [3H]‐thymidine incorporation significantly in both normal and cirrhotic rats. In cirrhotic rats, [3H]‐thymidine incorporation after HTX was significantly lower than in normal rats and administration of a combination of EGF and insulin after HTX enhanced [3H]‐thymidine incorporation. In conclusion, DNA synthesis 24 h after HTX is decreased in cirrhotic rats compared with normal rats and EGF supplementation with insulin accelerates DNA synthesis in hepatectomized cirrhotic rats. The data suggest that administration of combinations of exogenous hepatotrophic factors may play a useful role in the treatment of cirrhotic patients undergoing major hepatic resection.
部分肝切除后不同时间分离的原代培养物中的表皮生长因子和大鼠肝细胞增殖。
影响因子：
11.2
作者：
Francavilla,A;Ove,P;Polimeno,L;Sciascia,C;Coetzee,ML;Starzl,TE
通讯作者：
Starzl,TE
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Massagué,J
通讯作者：
Massagué,J