The nonprogressive motility of sperm populations from mice with a tw32 haplotype.
The nonprogressive motility of sperm populations from mice with a tw32 haplotype.
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具有 tw32 单倍型的小鼠精子群的非渐进运动。
DOI：
10.1002/j.1939-4640.1983.tb00738.x
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发表时间：
1983
影响因子：
--
通讯作者：
Olds-Clarke,P
中科院分区：
文献类型：
--
作者：
Olds-Clarke,P
我们之前曾报道，来自携带 tw32 单倍型的小鼠的附睾精子的净速度比来自同源 +/ + 雄性的精子低。基于精子无法在 4 秒内离开每边 200 μm 的立方体的非前向运动测试，目前已确定这些群体中非前向运动精子的频率在体外孵化 2 至 5 小时后超过 90%。在 Ca2+ < 0.1mM 的培养基中培养的精子群中从未出现非前向运动，尽管正常运动得到了很好的维持。在子宫精子群中也观察到非渐进性运动的频率较低，而在 +/+ 和 tw32/+ 男性的子宫输卵管精子中则观察到较高频率。这些观察结果表明，非前向运动可能是正常精子功能的一个组成部分，无论是在输卵管内的精子运输过程中，还是在受精过程中。
We have previously reported that epididymal spermatozoa from mice carrying a tw32haplotype have a lower net velocity than do spermatozoa from congenic +/ + males. Using a test for nonprogressive motility based on the inability of a spermatazoon to leave a cube 200 μm on each side in less than 4 seconds, the frequency of nonprogressively motile sperm in these populations now has been determined to be above 90% after 2 to 5 hours of incubationin vitro. The nonprogressive motility never appears in sperm populations incubated in media with < 0.1mM Ca2+, although normal motility is well‐maintained. Nonprogressive motility was also observed at low frequencies in uterine sperm populations, and at higher frequencies among uterotubal sperm from both +/+ and tw32/+ males. These observations suggest that nonprogressive motility may be a component of normal sperm function, either during sperm transport within the oviduct, or during fertilization.