Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8.
Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8.
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DOI：
10.1002/iid3.17
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发表时间：
2014-06
影响因子：
3.2
通讯作者：
Shimizu, Jun
中科院分区：
文献类型：
--
作者：
Shiheido, Hirokazu;Kitagori, Koji;Sasaki, Chiyomi;Kobayashi, Shio;Aoyama, Takane;Urata, Kozue;Oku, Takuma;Hirayama, Yoshitaka;Yoshitomi, Hiroyuki;Hikida, Masaki;Yoshifuji, Hajime;Mimori, Tsuneyo;Watanabe, Takeshi;Shimizu, Jun
BEN结构域蛋白3（BEND 3）没有跨膜区，定位于细胞质中，并且参与染色质功能和转录。我们在此鉴定了在其细胞表面上表达BEND 3的人T细胞的新亚群（BEND 3 + T细胞）。BEND 3 + T细胞由外周血中约3%的T细胞组成，存在于CD 4+和CD 8 + T细胞中，并且也在脐带血中观察到。通过TCR/CD 3复合物刺激BEND 3 + T细胞导致产生各种细胞因子;然而，BEND 3 + T细胞产生的IL-6和IL-8水平高于BEND 3 − T细胞。BEND 3 + T细胞的比例在一些炎症性疾病患者中也增加。总之，这些结果表明，BEND 3 + T细胞是T细胞在其细胞因子谱方面的新亚群。对BEND 3 + T细胞的进一步分析可能对理解人类T细胞免疫学具有重要意义。
BEN domain-containing protein 3 (BEND3) has no transmembrane region, is localized in the cytoplasm, and is involved in chromatin function and transcription. We here identified a novel subpopulation of human T cells that expressed BEND3 on their cell surface (BEND3+ T cells). BEND3+ T cells consisted of approximately 3% of T cells in the peripheral blood, were present in both CD4+ and CD8+ T cells, and were also observed in cord blood. The stimulation of BEND3+ T cells through the TCR/CD3 complex led to the production of various kinds of cytokines; however, the levels of IL-6 and IL-8 produced by BEND3+ T cells were higher than those by BEND3− T cells. The proportion of BEND3+ T cells was also increased in some patients with inflammatory diseases. Taken together, these results indicate that BEND3+ T cells are a new subpopulation of T cells in terms of their cytokine profile. Further analyses on BEND3+ T cells may be of importance and useful in understanding human T cell immunology.