Metagenomic analysis of an ecological wastewater treatment plant's microbial communities and their potential to metabolize pharmaceuticals.
Metagenomic analysis of an ecological wastewater treatment plant's microbial communities and their potential to metabolize pharmaceuticals.
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DOI：
10.12688/f1000research.9157.1
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Leduc M
中科院分区：
文献类型：
--
作者：
Balcom IN;Driscoll H;Vincent J;Leduc M
在世界各地的饮用水、地下水、地表水和土壤中检测到药物和其他微污染物。即使在需要废水处理的地方，它们也可以在饮用水威尔斯、市政供水和农业土壤中找到。很明显，传统的废水处理技术无法应对全球淡水供应日益增加的压力。为此，开发了具有成本效益的生态废水处理技术。为了确定生态污水处理厂（WWTPs）中微污染物的去除是否由植物-微生物相互作用促进，如已报道的其他柠檬酸类外源性物质，通过全宏基因组测序分析生长在植物根表面的生物膜微生物群落，并与废水中的微生物群落进行比较。在这项研究中，药物和个人护理产品（PPCPs）的浓度进行了量化，在每个处理池的生态污水处理厂处理人类废水在高速公路休息站和游客中心在佛蒙特州。检测到的PPCPs的浓度远大于传统的污水处理厂可能是由于现场再循环的废水报告的值。在缺氧处理池中观察到PPCPs浓度的最大减少，其中芽孢杆菌占主导地位的生物膜群落。苯甲酸酯降解是整个系统中最丰富的异生物质代谢类别。总的来说，居住在废水中的微生物群落的分类和代谢比浸没植物根生物膜更多样化。然而，更大的异质性和更高的相对丰度的异生物质代谢基因观察到的根生物膜。
Pharmaceuticals and other micropollutants have been detected in drinking water, groundwater, surface water, and soil around the world. Even in locations where wastewater treatment is required, they can be found in drinking water wells, municipal water supplies, and agricultural soils. It is clear conventional wastewater treatment technologies are not meeting the challenge of the mounting pressures on global freshwater supplies. Cost-effective ecological wastewater treatment technologies have been developed in response. To determine whether the removal of micropollutants in ecological wastewater treatment plants (WWTPs) is promoted by the plant-microbe interactions, as has been reported for other recalcitrant xenobiotics, biofilm microbial communities growing on the surfaces of plant roots were profiled by whole metagenome sequencing and compared to the microbial communities residing in the wastewater. In this study, the concentrations of pharmaceuticals and personal care products (PPCPs) were quantified in each treatment tank of the ecological WWTP treating human wastewater at a highway rest stop and visitor center in Vermont. The concentrations of detected PPCPs were substantially greater than values reported for conventional WWTPs likely due to onsite recirculation of wastewater. The greatest reductions in PPCPs concentrations were observed in the anoxic treatment tank where Bacilli dominated the biofilm community. Benzoate degradation was the most abundant xenobiotic metabolic category identified throughout the system. Collectively, the microbial communities residing in the wastewater were taxonomically and metabolically more diverse than the immersed plant root biofilm. However, greater heterogeneity and higher relative abundances of xenobiotic metabolism genes was observed for the root biofilm.