Acidithiobacillus ferrooxidans's comprehensive model driven analysis of the electron transfer metabolism and synthetic strain design for biomining applications.
Acidithiobacillus ferrooxidans's comprehensive model driven analysis of the electron transfer metabolism and synthetic strain design for biomining applications.
复制标题
酸硫代杆菌铁氧化物对电子转移代谢和合成应变设计的综合模型驱动分析。
DOI：
10.1016/j.meteno.2016.03.003
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发表时间：
2016-12
影响因子：
5.2
通讯作者：
Asenjo JA
中科院分区：
文献类型：
--
作者：
Campodonico MA;Vaisman D;Castro JF;Razmilic V;Mercado F;Andrews BA;Feist AM;Asenjo JA
酸性氧化亚铁硫杆菌是一种革兰氏阴性趋化石质自养γ-变形杆菌。它通常在外部pH值为2的环境下生长，利用氧氧化亚铁离子，产生铁离子和水，同时从环境中固定二氧化碳。A.氧化亚铁对生物采矿和环境应用非常有兴趣，因为它可以处理矿物矿石并减轻采矿过程产生的负面环境后果。在这项研究中，首次生成了a.f erferrooxidans ATCC 23270 （iMC507）的基因组尺度代谢重建。共有587个代谢和转运/交换反应、507个基因和573个代谢物组织在超过42个子系统中被纳入模型。基于一种新的遗传算法，结合通量平衡分析、化学渗透理论和生理数据，估计了三种不同电子供体在有氧化石化自养条件下许多酶的质子易位化学计量学和维持参数。此外，详细的电子转移和碳通量分布在化学岩石自养生长中使用亚铁离子，四硫酸盐和硫代硫酸盐。最后，计算了134种生长偶联设计，使细胞外多糖的生产成为可能。iMC507是一个知识库，用于总结和分类a.f erferrooxidans的现有信息，并从综合模型驱动的角度对酸化硫杆菌和类似物种进行理解和工程设计，用于生物矿化应用。首次基因组规模的酸性氧化亚铁硫杆菌代谢网络重建。基于基因组尺度的质子易位化学计量学估计。胞外多糖生长偶联生产的菌株设计。
Acidithiobacillus ferrooxidans is a gram-negative chemolithoautotrophic γ-proteobacterium. It typically grows at an external pH of 2 using the oxidation of ferrous ions by oxygen, producing ferric ions and water, while fixing carbon dioxide from the environment. A. ferrooxidans is of great interest for biomining and environmental applications, as it can process mineral ores and alleviate the negative environmental consequences derived from the mining processes. In this study, the first genome-scale metabolic reconstruction of A. ferrooxidans ATCC 23270 was generated (iMC507). A total of 587 metabolic and transport/exchange reactions, 507 genes and 573 metabolites organized in over 42 subsystems were incorporated into the model. Based on a new genetic algorithm approach, that integrates flux balance analysis, chemiosmotic theory, and physiological data, the proton translocation stoichiometry for a number of enzymes and maintenance parameters under aerobic chemolithoautotrophic conditions using three different electron donors were estimated. Furthermore, a detailed electron transfer and carbon flux distributions during chemolithoautotrophic growth using ferrous ion, tetrathionate and thiosulfate were determined and reported. Finally, 134 growth-coupled designs were calculated that enables Extracellular Polysaccharide production. iMC507 serves as a knowledgebase for summarizing and categorizing the information currently available for A. ferrooxidans and enables the understanding and engineering of Acidithiobacillus and similar species from a comprehensive model-driven perspective for biomining applications. First genome scale reconstruction metabolic network of Acidithiobacillus ferrooxidans. Genome scale based proton translocation stoichiometry estimation. Strain designs for Extracellular Polysaccharide growth-coupling production.