Exposure to wood smoke particles produces inflammation in healthy volunteers
Exposure to wood smoke particles produces inflammation in healthy volunteers
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DOI：
10.1136/oem.2011.065276
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发表时间：
2012-03-01
影响因子：
4.9
通讯作者：
Rappold, Ana
中科院分区：
文献类型：
--
作者：
Ghio, Andrew J.;Soukup, Joleen M.;Rappold, Ana
目标 人类接触木材烟雾颗粒 (WSP) 通过室内空气质量变化、野火暴露、生物质燃烧和空气污染对人类健康产生影响。这项调查测试了这样的假设：暴露于 WSP 的健康志愿者会表现出肺部和全身炎症的证据。方法 10 名志愿者暴露于过滤空气，3 周或更长时间后，暴露于 WSP。每次暴露包括交替 15 分钟的运动和 15 分钟的休息，总持续时间为 2 小时。通过在电子元件上加热橡木原木产生木烟，然后输送到暴露室。志愿者测量的终点包括症状、肺功能测试、心率变异性和复极测量、血液指数以及支气管肺泡灌洗过程中获得的细胞和液体分析。 结果 使用过滤器上收集的颗粒质量测量 10 次暴露于空气和 WSP 的平均颗粒质量，发现分别低于可检测限和 485 +/- 84 mu g/m(3)（平均值 +/- SD）。接触 WSP 后，症状发生率或肺功能均没有变化。暴露于木烟20小时后，血液检测显示中性粒细胞百分比增加，支气管和支气管肺泡灌洗显示中性粒细胞流入。结论我们得出的结论是，健康志愿者暴露于WSP可能与全身和肺部炎症的证据有关。
Objectives Human exposure to wood smoke particles (WSP) impacts on human health through changes in indoor air quality, exposures from wild fires, burning of biomass and air pollution. This investigation tested the postulate that healthy volunteers exposed to WSP would demonstrate evidence of both pulmonary and systemic inflammation.Methods Ten volunteers were exposed to filtered air and, 3 weeks or more later, WSP. Each exposure included alternating 15 min of exercise and 15 min of rest for a total duration of 2 h. Wood smoke was generated by heating an oak log on an electric element and then delivered to the exposure chamber. Endpoints measured in the volunteers included symptoms, pulmonary function tests, measures of heart rate variability and repolarisation, blood indices and analysis of cells and fluid obtained during bronchoalveolar lavage.Results Mean particle mass for the 10 exposures to air and WSP was measured using the mass of particles collected on filters and found to be below the detectable limit and 485 +/- 84 mu g/m(3), respectively (mean +/- SD). There was no change in either symptom prevalence or pulmonary function with exposure to WSP. At 20 h after wood smoke exposure, blood tests demonstrated an increased percentage of neutrophils, and bronchial and bronchoalveolar lavage revealed a neutrophilic influx.Conclusions We conclude that exposure of healthy volunteers to WSP may be associated with evidence of both systemic and pulmonary inflammation.