Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam
Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam
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DOI：
10.1016/j.foodchem.2013.10.094
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发表时间：
2014-04-15
期刊：
影响因子：
8.8
通讯作者：
Osako, Kazufumi
中科院分区：
文献类型：
--
作者：
Le Thi Minh Thuy;Okazaki, Emiko;Osako, Kazufumi
从日本和越南的蜥蜴鱼（Saurida spp.）和竹荚鱼（Trachurus japonicus）以及日本的灰鲻鱼（Mugil cephalis）、飞鱼（Cypselurus melanurus）和黄背鲷（Dentex tumifrons）的鳞片中成功提取了酸溶性胶原蛋白（ASC）。 ASC 产量约为 0.43-1.5%（以干重计），具体取决于物种。 SDS-PAGE图谱显示ASC是I型胶原蛋白，由两条不同的α链α1和α2以及β成分组成。越南竹荚鱼ASC 的亚氨基酸含量高于日本竹荚鱼ASC。 ASC 变性温度 (T-d) 范围为 26 至 29 摄氏度，具体取决于鱼类种类和亚氨基酸含量 (p < 0.01)。在 pH 1-3 时观察到各个胶原蛋白的最大溶解度。无论鱼类类型如何，当 NaCl 浓度 >0.4 M 时，胶原蛋白溶解度都会急剧下降。 (C) 2013 Elsevier Ltd. 保留所有权利。
Acid-soluble collagen (ASC) was successfully extracted from the scales of lizard fish (Saurida spp.) and horse mackerel (Trachurus japonicus) from Japan and Vietnam and grey mullet (Mugil cephalis), flying fish (Cypselurus melanurus) and yellowback seabream (Dentex tumifrons) from Japan. ASC yields were about 0.43-1.5% (on a dry weight basis), depending on the species. The SDS-PAGE profile showed that the ASCs were type I collagens, and consisted of two different a chains, alpha 1 and alpha 2, as well as a beta component. ASC of horse mackerel from Vietnam contained a higher imino acid level than that from Japan. ASC denaturation temperature (T-d) ranged from 26 to 29 degrees C, depending on fish species and imino acid content (p < 0.01). Maximal solubility of individual collagens was observed at pHs 1-3. Collagen solubility decreased sharply at NaCl concentrations >0.4 M, regardless of fish type. (C) 2013 Elsevier Ltd. All rights reserved.