Novel circRNAs from cashmere goats: Discovery, integrated regulatory network, and their putative roles in the regeneration and growth of secondary hair follicles
Novel circRNAs from cashmere goats: Discovery, integrated regulatory network, and their putative roles in the regeneration and growth of secondary hair follicles
复制标题
绒山羊的新型 circRNA：发现、整合调控网络及其在次级毛囊再生和生长中的假定作用
DOI：
10.17221/179/2021-cjas
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发表时间：
2022-01-01
影响因子：
1.2
通讯作者：
Bai, Wenlin
中科院分区：
文献类型：
--
作者：
Shen, Jincheng;Wang, Yanru;Bai, Wenlin
环状RNA(CircRNAs)是一种非编码RNA，具有不同于其他类型的RNA分子的共价闭合结构。有人认为CircRNAs与绒山羊二次毛囊的发育有关。在本研究中，从绒山羊的皮肤组织中共鉴定出21个新的CircRNA。其中9个CircRNA在绒山羊生长期皮肤组织中的表达显著高于在绒山羊皮肤组织中的表达。通过生物信息学分析，揭示了生长期上调的9个CircRNAs与Wnt信号通路、转化生长因子-β信号通路、mTOR信号通路、MAPK信号通路和轴突导向等相关信号通路之间的复杂调控关系。此外，对9个生长期上调的CircRNAs及其宿主基因在绒山羊生长期的表达模式进行了分析，表明它们在绒山羊生长期生长期的再生和生长中具有潜在的功能作用。我们的研究结果为阐明绒山羊绒纤维再生和生长的分子调控机制提供了新的有意义的信息，这对于人工调控绒山羊生长以提高绒山羊产量具有重要意义。
Circular RNAs (circRNAs) were identified as noncoding RNAs with covalently closed structures differing from other types of RNA molecules. It was thought that circRNAs were implicated in the development of secondar y hair follicles (SHFs) of cashmere goats. In this investigation, a total of 21 novel circRNAs were identified from the skin tissue of cashmere goats. Of them, nine circRNAs were found to be significantly higher in expression at anagen skin tissue than those at telogen of cashmere goats. Based on bioinformatics analysis, a complicated regulatory relationship was revealed among the nine upregulated circRNAs at anagen with related signalling pathways, such as Wnt signalling pathway, TGF-beta signalling pathway, mTOR signalling pathway, MAPK signalling pathway, and axon guidance. Further, the expression pattern analysis of the nine upregulated circRNAs at anagen along with their host genes in SHFs suggested their potential functional roles in the regeneration and growth of anagen SHFs of cashmere goats. Our results provided novel significant information for elucidating the molecular regulatory mechanisms understanding the regeneration and growth of SHFs in cashmere goats, which will be essential for artificially regulating the growth of cashmere to increase cashmere yield.