The consequence of biologic graft processing on blood interface biocompatibility and mechanics.
The consequence of biologic graft processing on blood interface biocompatibility and mechanics.
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DOI：
10.1007/s13239-015-0221-2
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发表时间：
2015-09
影响因子：
1.8
通讯作者：
McFetridge PS
中科院分区：
文献类型：
--
作者：
Van de Walle AB;Uzarski JS;McFetridge PS
处理离体衍生组织以降低免疫原性是产生用于血管重建的生物复合材料的有效方法。由于小直径血管移植物对闭塞事件的敏感性，需要详细分析移植物处理对移植物通畅性关键参数（如外周细胞粘附和壁力学）的影响。分离的人脐静脉切片用作模型同种异体血管支架，其用以下任一种处理：1.十二烷基硫酸钠（SDS），2.乙醇/丙酮（EtOAc），或3.戊二醛（Glu）。通过单轴拉伸试验评估材料力学变化。外周细胞粘附到不透明的移植材料进行了评估，使用一个创新的流动室，允许直接观察血液移植界面下的生理剪切条件。所有处理均改变了移植物的拉伸应变和刚度特性，生理模量值从Glu 240±12 kPa降至SDS 210±6 kPa和EtAc 140±3 kPa，P<.001。相对于戊二醛处理，中性粒细胞与脱细胞移植物的粘附增加，SDS或EtAc处理之间未观察到统计学差异。早期血小板粘附（%表面覆盖率）显示三种治疗之间无统计学差异;然而，与EtOAc或Glu相比，SDS支架上血小板聚集体的定量显著更高。应用于脐静脉支架的组织处理策略显示出改变结构力学和细胞粘附特性，其中相对于SDS处理的样品，EtAc处理减少血栓形成事件。这种方法允许对临床相关移植材料进行时间和成本有效的预筛选，以评估初始细胞反应性。
Processing ex vivo derived tissues to reduce immunogenicity is an effective approach to create biologically complex materials for vascular reconstruction. Due to the sensitivity of small diameter vascular grafts to occlusive events, the effect of graft processing on critical parameters for graft patency, such as peripheral cell adhesion and wall mechanics, requires detailed analysis. Isolated human umbilical vein sections were used as model allogenic vascular scaffolds that were processed with either: 1. sodium dodecyl sulfate (SDS), 2. ethanol/acetone (EtAc), or 3. glutaraldehyde (Glu). Changes in material mechanics were assessed via uniaxial tensile testing. Peripheral cell adhesion to the opaque grafting material was evaluated using an innovative flow chamber that allows direct observation of the blood-graft interface under physiological shear conditions. All treatments modified the grafts tensile strain and stiffness properties, with physiological modulus values decreasing from Glu 240±12 kPa to SDS 210±6 kPa and EtAc 140±3 kPa, P<.001. Relative to glutaraldehyde treatments, neutrophil adhesion to the decellularized grafts increased, with no statistical difference observed between SDS or EtAc treatments. Early platelet adhesion (% surface coverage) showed no statistical difference between the three treatments; however, quantification of platelet aggregates was significantly higher on SDS scaffolds compared to EtAc or Glu. Tissue processing strategies applied to the umbilical vein scaffold were shown to modify structural mechanics and cell adhesion properties, with the EtAc treatment reducing thrombotic events relative to SDS treated samples. This approach allows time and cost effective prescreening of clinically relevant grafting materials to assess initial cell reactivity.