A Genetically Heterogeneous Rat Model with Divergent Mitochondrial Genomes.
A Genetically Heterogeneous Rat Model with Divergent Mitochondrial Genomes.
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DOI：
10.1093/gerona/glad056
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发表时间：
2023-05-11
影响因子：
5.1
通讯作者：
Richardson, Arlan
中科院分区：
文献类型：
--
作者：
Sathiaseelan, Roshini;Ahn, Bumsoo;Stout, Michael B.;Logan, Sreemathi;Wanagat, Jonathan;Nguyen, Hoang Van M.;Hord, Norman G.;Vandiver, Amy R.;Selvarani, Ramasamy;Ranjit, Rojina;Yarbrough, Hannah;Masingale, Anthony;Miller, Benjamin F.;Wolf, Roman F.;Austad, Steven N.;Richardson, Arlan
We generated a genetically heterogenous rat model by a 4-way cross strategy using 4 inbred strains (Brown Norway [BN], Fischer 344 [F344], Lewis [LEW], and Wistar Kyoto [KY]) to provide investigators with a highly genetically diverse rat model from commercially available inbred rats. We made reciprocal crosses between males and females from the 2 F1 hybrids to generate genetically heterogeneous rats with mitochondrial genomes from either the BN (OKC-HETB, a.k.a “B” genotype) or WKY (OKC-HETW a.k.a “W” genotype) parental strains. These two mitochondrial genomes differ at 94 nucleotides, more akin to human mitochondrial genome diversity than that available in classical laboratory mouse strains. Body weights of the B and W genotypes were similar. However, mitochondrial genotype antagonistically affected grip strength and treadmill endurance in females only. In addition, mitochondrial genotype significantly affected multiple responses to a high-fat diet (HFD) and treatment with 17α-estradiol. Contrary to findings in mice in which males only are affected by 17α-estradiol supplementation, female rats fed a HFD beneficially responded to 17α-estradiol treatment as evidenced by declines in body mass, adiposity, and liver mass. Male rats, by contrast, differed in a mitochondrial genotype-specific manner, with only B males responding to 17α-estradiol treatment. Mitochondrial genotype and sex differences were also observed in features of brain-specific antioxidant response to a HFD and 17α-estradiol as shown by hippocampal levels of Sod2 acetylation, JNK, and FoxO3a. These results emphasize the importance of mitochondrial genotype in assessing responses to putative interventions in aging processes.
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影响因子：
2.9
作者：
Yi KD;Perez E;Yang S;Liu R;Covey DF;Simpkins JW
通讯作者：
Simpkins JW
影响因子：
7.8
作者：
Garratt M;Bower B;Garcia GG;Miller RA
通讯作者：
Miller RA
DOI：
10.1042/bj20130029
发表时间：
2013-10-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Fetterman JL;Zelickson BR;Johnson LW;Moellering DR;Westbrook DG;Pompilius M;Sammy MJ;Johnson M;Dunham-Snary KJ;Cao X;Bradley WE;Zhang J;Wei CC;Chacko B;Schurr TG;Kesterson RA;Dell'italia LJ;Darley-Usmar VM;Welch DR;Ballinger SW
通讯作者：
Ballinger SW
影响因子：
7.8
作者：
Harrison DE;Strong R;Allison DB;Ames BN;Astle CM;Atamna H;Fernandez E;Flurkey K;Javors MA;Nadon NL;Nelson JF;Pletcher S;Simpkins JW;Smith D;Wilkinson JE;Miller RA
通讯作者：
Miller RA
归因于17α-雌二醇（一种延伸寿命的化合物）归因于雌激素受体α介导的。
影响因子：
7.7
作者：
Mann SN;Hadad N;Nelson Holte M;Rothman AR;Sathiaseelan R;Ali Mondal S;Agbaga MP;Unnikrishnan A;Subramaniam M;Hawse J;Huffman DM;Freeman WM;Stout MB
通讯作者：
Stout MB