Physical Mechanisms Shaping the Drosophila Dorsoventral Compartment Boundary
Physical Mechanisms Shaping the Drosophila Dorsoventral Compartment Boundary
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DOI：
10.1016/j.cub.2012.03.070
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发表时间：
2012-06-05
期刊：
影响因子：
9.2
通讯作者：
Dahmann, Christian
中科院分区：
文献类型：
--
作者：
Aliee, Maryam;Roeper, Jens-Christian;Dahmann, Christian
背景资料：通过直的和尖锐的隔室边界分离具有不同身份和命运的细胞对于动物发育期间的生长和模式形成是重要的。然而，尚未完全理解的物理机制，塑造室边界。结果：我们联合收割机理论和定量实验相结合，探讨不同的机制，以塑造室边界的作用。我们的理论工作表明，细胞伸长所产生的各向异性应力，细胞增殖率，细胞分裂的方向，和细胞键张力都有明显的影响，在组织生长的隔室边界的形态。我们的实验使用发展中的果蝇翅膀显示，背腹侧隔室边界的粗糙度是动态的，它在发展过程中减少。通过测量组织弛豫响应于激光消融的细胞债券在不同的发展时间，我们表明，减少边界粗糙度与增加细胞键张力沿着室边界。最后，通过使用实验确定的值为细胞键张力，细胞伸长和细胞分裂的方向在模拟组织生长的偏见，我们可以重现的背腹侧隔室边界形状的时间演变的主要特征。结论：局部增加的细胞键张力沿着边界以及全球各向异性的组织有助于塑造细胞网络的边界。我们提出了一个简单的方案，结合时间依赖性的细胞键张力的边界，定向细胞分裂，和细胞伸长的组织中，可以占的背腹侧隔室边界的形状的动力学的主要特征。
Background: Separating cells with distinct identities and fates by straight and sharp compartment boundaries is important for growth and pattern formation during animal development. The physical mechanisms shaping compartment boundaries, however, are not fully understood.Results: We combine theory and quantitative experiments to investigate the roles of different mechanisms to shape compartment boundaries. Our theoretical work shows that cell elongation created by anisotropic stress, cell proliferation rate, orientation of cell division, and cell bond tension all have distinct effects on the morphology of compartment boundaries during tissue growth. Our experiments using the developing Drosophila wing reveal that the roughness of the dorsoventral compartment boundary is dynamic and that it decreases during development. By measuring tissue relaxation in response to laser ablation of cell bonds at different developmental times, we demonstrate that decreased boundary roughness correlates with increased cell bond tension along the compartment boundary. Finally, by using experimentally determined values for cell bond tension, cell elongation and bias in orientation of cell division in simulations of tissue growth, we can reproduce the main features of the time evolution of the dorsoventral compartment boundary shape.Conclusions: Local increase of cell bond tension along the boundary as well as global anisotropies in the tissue contribute to shaping boundaries in cell networks. We propose a simple scenario that combines time-dependent cell bond tension at the boundary, oriented cell division, and cell elongation in the tissue that can account for the main features of the dynamics of the shape of the dorsoventral compartment boundary.