Effect of an emulsifying surfactant on diesel degradation by cultures exhibiting inducible cell surface hydrophobicity
Effect of an emulsifying surfactant on diesel degradation by cultures exhibiting inducible cell surface hydrophobicity
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DOI：
10.1002/jctb.1753
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发表时间：
2007-11-01
影响因子：
3.4
通讯作者：
Mukherji, Suparna
中科院分区：
文献类型：
--
作者：
Mohanty, Gita;Mukherji, Suparna
背景：石油碳氢化合物在环境中的积累往往归因于其固有的缓慢降解速度。进行这项研究，以确定是否柴油生物降解的文化表现出诱导细胞表面疏水性可以通过添加化学表面活性剂促进乳化增强。结果：在2倍于临界胶束浓度（CMC）的Triton X-100存在下，5天内对全柴油的降解程度是不加表面活性剂时15天的1.6-1.8倍。柴油范围分辨峰（DRRP，代表正构烷烃和支链烷烃）的总降解的增强是1.76和1.26倍。aurantiacum和B.洋葱培养物。尽管观察到所有正构烷烃组分的衰变率均有所增强，但C19、C25和降植烷的衰变率最为显着。结论：对于表现出直接界面吸收的培养物，本文发现的乳化表面活性剂的有益效果与早期研究相反，报告了由于细胞从油/水界面脱离而产生的有害影响。表面活性剂的加入可能具有显著的潜力，在提高更多的难降解成分的降解。(C)2007年化学工业协会
Background: Accumulation of petroleum hydrocarbons in the environment is often attributed to inherently slow degradation rates. This study was conducted to determine if diesel biodegradation by cultures exhibiting inducible cell surface hydrophobicity could be enhanced by addition of a chemical surfactant promoting emulsification. Experiments were designed to determine quantitatively the enhancement in diesel degradation caused by Triton X-100 for two bacterial cultures, Exiguobacterium aurantiacum and Burkholderia cepacia.Results: In the presence of Triton X-100 at twice the critical micelle concentration (CMC) the extent of degradation in whole diesel in 5 days was 1.6-1.8 times that observed over 15 days with no surfactant. The enhancement in overall degradation of diesel range resolved peaks (DRRP, representing n-alkanes and branched alkanes) was 1.76 and 1.26 times for E. aurantiacum and B. cepacia cultures respectively. Although an enhancement in decay rate was observed for all n-alkane components, it was most significant for C19, C25 and pristane.Conclusion: For cultures exhibiting direct interfacial uptake, the beneficial effect of an emulsifying surfactant found here is in contrast to earlier studies reporting a detrimental effect due to detachment of cells from the oil/water interface. Surfactant addition may have significant potential in enhancing degradation of the more recalcitrant components. (C) 2007 Society of Chemical Industry