COLLISION-INDUCED DISSOCIATION OF ALKALI-METAL CATIONIZED AND PERMETHYLATED OLIGOSACCHARIDES - INFLUENCE OF THE COLLISION ENERGY AND OF THE COLLISION GAS FOR THE ASSIGNMENT OF LINKAGE POSITION
COLLISION-INDUCED DISSOCIATION OF ALKALI-METAL CATIONIZED AND PERMETHYLATED OLIGOSACCHARIDES - INFLUENCE OF THE COLLISION ENERGY AND OF THE COLLISION GAS FOR THE ASSIGNMENT OF LINKAGE POSITION
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DOI：
10.1016/1044-0305(93)85081-8
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发表时间：
1993-03-01
影响因子：
3.2
通讯作者：
DEHOFFMANN, E
中科院分区：
文献类型：
--
作者：
LEMOINE, J;FOURNET, B;DEHOFFMANN, E
串联质谱已被用来研究碰撞诱导的[M + Na]+离子的全甲基化寡糖的分解。它示出，在一个化合物中的许多连接位置可以由存在或不存在，在一个单一的光谱中，产生的两个环键的裂解的特定碎片离子，这种离子的产量强烈地依赖于碰撞能量和碰撞气体的性质。与单锂化天然低聚糖的行为相反，钠化和全甲基化低聚糖样品在低能量下的碰撞诱导分解导致糖苷键的优先裂解。在高碰撞能量下，由多个键断裂形成的碎片离子大大增强，尤其是当氦被氩气取代作为碰撞气体时。此外，氩气是诱导前体离子碎裂的更有效的碰撞气体。作为该方法应用的一个例子，描述了N-聚糖的共同核心中1 -> 3和1 -> 6-连接的甘露糖残基之间的区分。
Tandem mass spectrometry has been used to study the collision-induced decomposition of [M + Na]+ ions of permethylated oligosaccharides. It is shown that many linkage positions in one compound may be determined by the presence or absence, in a single spectrum, of specific fragment ions that arise from the cleavage of two ring bonds and that the yield of such ions depends strongly on the collision energy and nature of the collision gas. In contrast to the behavior of monolithiated native oligosaccharides, the collision-induced decomposition of the sodiated and permethylated oligosaccharide samples at low energy leads to preferential cleavage of glycosidic linkages. At high collision energies, the fragment ions formed by cleavage of more than one bond are greatly enhanced, especially when helium is replaced by argon as the collision gas. Furthermore, argon is the more efficient collision gas in inducing fragmentation of the precursor ions. As an example of the application of this method, the discrimination between the 1 --> 3 and 1 --> 6-linked mannose residues in the common core of N-glycans is described.