Both oxidative and nitrosative stress are associated with muscle wasting in tumour-bearing rats
Both oxidative and nitrosative stress are associated with muscle wasting in tumour-bearing rats
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DOI：
10.1016/j.febslet.2005.02.017
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发表时间：
2005-03-14
期刊：
影响因子：
3.5
通讯作者：
Argilés, JM
中科院分区：
文献类型：
--
作者：
Barreiro, E;de la Puente, B;Argilés, JM
活性氧和氮物质（ROS和RNS）已被提出作为癌症诱导的恶病质的机制。在这项研究中，我们使用Western印迹分析评估总蛋白羰基化（2，4-二硝基苯肼测定），丙二醛（MDA-）和2-羟基-4-壬烯醛（HNE-）蛋白加合物，锰超氧化物歧化酶（MnSOD），过氧化氢酶，血红素加氧酶-1（HO-1）和3-硝基酪氨酸形成的水平，在腓肠肌的大鼠轴承吉田AH-130肝癌。在肌肉中的荷瘤动物，蛋白质羰基化作为测量总水平的羰基形成和HNE和MDA-蛋白质加合物，和蛋白质酪氨酸硝化显着大于对照肌肉。蛋白质水平的抗氧化酶锰超氧化物歧化酶，过氧化氢酶，HO-1没有显着修改大鼠恶病质肌肉相比，控制。抗氧化酶在中和过量ROS产生中的无效性可能是蛋白质氧化标记物升高的原因，并且是癌症诱导的恶病质中氧化应激和亚硝化应激发展的原因。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Reactive oxygen and nitrogen species (ROS and RNS) have been proposed as mechanisms of cancer-induced cachexia. In this study, we assessed using Western blot analysis the levels of total protein carbonylation (2,4-dinitrophenylhydrazine assay), both malondialdehyde- (MDA-) and 2-hydroxy-4-nonenal- (HNE-) protein adducts, Mn-superoxide dismutase (MnSOD), catalase, heme oxygenase-1 (HO-1) and 3-nitrotyrosine formation in gastrocnemius muscles of rats bearing the Yoshida AH-130 hepatoma. In the muscles of the tumour-bearing animals, protein carbonylation as measured by total levels of carbonyl group formation and both HNE and MDA-protein adducts, and protein tyrosine nitration were significantly greater than in control muscles. Protein levels of the antioxidant enzymes Mn-SOD, catalase, and HO-1 were not significantly modified in the rat cachectic muscles compared to controls. The inefficiency of the antioxidant enzymes in neutralizing excessive ROS production may account for elevated markers of protein oxidation and be responsible for the development of both oxidative and nitrosative stress in cancer-induced cachexia. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.