NEUROIMMUNE COMMUNICATION IN THE SUBMUCOUS PLEXUS OF GUINEA-PIG COLON AFTER INFECTION WITH TRICHINELLA-SPIRALIS
NEUROIMMUNE COMMUNICATION IN THE SUBMUCOUS PLEXUS OF GUINEA-PIG COLON AFTER INFECTION WITH TRICHINELLA-SPIRALIS
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DOI：
10.1016/0016-5085(94)90798-6
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发表时间：
1994-12-01
期刊：
影响因子：
29.4
通讯作者：
WOOD, JD
中科院分区：
文献类型：
--
作者：
FRIELING, T;PALMER, JM;WOOD, JD
背景/目的：胃肠道超敏反应中的肠神经免疫通讯包括肥大细胞检测抗原和向肠神经系统释放化学信息。本研究的目的是分析早期感染旋毛虫的动物暴露于旋毛虫抗原时结肠粘膜下神经丛神经元的电和突触行为。方法：应用微电极对豚鼠远端结肠粘膜下神经元进行旋毛虫抗原的记录。结果：致敏动物的神经元比对照组更容易兴奋。过度兴奋性被认为是自发动作电位放电和对去极化电流或暴露于乙酰胆碱的重复放电的更大概率。组胺能拮抗剂的应用逆转了增强的兴奋性，提示内源性释放组胺是一个负责任的因素。抗原暴露仅在致敏动物中增加神经元兴奋性并抑制快速胆碱能突触的尼古丁传递。西咪替丁阻断了对兴奋性的影响，但未阻断突触前抑制作用。结论：粘膜肥大细胞和肠神经系统之间的信号通路参与了致敏抗原引起的结肠过敏反应。组胺是沟通通路中的旁分泌信号。
Background/Aims: Enteric neuroimmune communication in gastrointestinal hypersensitivity responses includes antigen detection by mast cells and release of chemical messages to the enteric nervous system. The aim of this study was to analyze the electrical and synaptic behavior of neurons in the colonic submucous plexus during exposure to Trichinella spiralis antigen in animals infected earlier with the parasite. Methods: Microelectrodes were used to record in submucous neurons of guinea pig distal colon during application of Trichinella antigen. Results: Neurons in sensitized animals were more excitable than in controls. Hyperexcitability was seen as a greater probability of spontaneous action potential discharge and repetitive firing to depolarizing current or exposure to acetylcholine. Application of histaminergic antagonists reversed the augmented excitability, suggesting endogenously released histamine as a responsible factor. Antigenic exposure increased neuronal excitability and suppressed nicotinic transmission at fast cholinergic synapses only in sensitized animals. Effects on excitability, but not pre synaptic inhibitory effects, were blocked by cimetidine. Conclusions: Signaling between mucosal mast cells and the enteric nervous system is involved in colonic anaphylactic responses to sensitizing antigens. Histamine is a paracrine signal in the communication pathway.