Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications
Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications
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DOI：
10.1016/j.bmcl.2009.12.077
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发表时间：
2010-02-01
影响因子：
2.7
通讯作者：
Liu, Wenshe
中科院分区：
文献类型：
--
作者：
Huang, Ying;Wan, Wei;Liu, Wenshe
通过进化的吡咯烷基-tRNA合成酶/PylT对，2-氨基-8-氧农酸在大肠杆菌中被基因整合到蛋白质中。在接近生理pH的温和条件下，直接利用该氨基酸中酮基团与含肼和烷氧胺的化合物的独有反应性来定位标记蛋白质显示出非常高的效率。爱思唯尔有限公司出版。
2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency. Published by Elsevier Ltd.