Molecular and electrophysiological evidence for net synaptic potentiation in wake and depression in sleep
Molecular and electrophysiological evidence for net synaptic potentiation in wake and depression in sleep
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DOI：
10.1038/nn2035
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发表时间：
2008-02-01
影响因子：
25
通讯作者：
Tononi, Giulio
中科院分区：
文献类型：
--
作者：
Vyazovskiy, Vladyslav V.;Cirelli, Chiara;Tononi, Giulio
在清醒时发生的可塑性变化有助于记忆的获取和巩固。对于某些记忆，进一步巩固需要睡眠，但清醒和睡眠期间的可塑性过程是否不同尚不清楚。我们发现，在大鼠皮层和海马中，含glur1的AMPA受体（AMPAR）水平在清醒时高，在睡眠时低，AMPAR、CamKII和GSK3 β磷酸化状态的变化与清醒时的突触增强和睡眠时的抑郁一致。此外，皮层诱发反应的斜率和振幅在清醒后增加，在睡眠后减少，并与慢波活动的变化相关，慢波活动是睡眠压力的标志。突触强度的分子和电生理指标的变化在很大程度上与一天中的时间无关。最后，皮层长期增强在睡眠后容易被诱导，但在清醒后不容易被诱导。因此，清醒似乎与净突触增强有关，而睡眠可能有利于整体突触抑制，从而保持突触强度的整体平衡。
Plastic changes occurring during wakefulness aid in the acquisition and consolidation of memories. For some memories, further consolidation requires sleep, but whether plastic processes during wakefulness and sleep differ is unclear. We show that, in rat cortex and hippocampus, GluR1-containing AMPA receptor ( AMPAR) levels are high during wakefulness and low during sleep, and changes in the phosphorylation states of AMPARs, CamKII and GSK3 beta are consistent with synaptic potentiation during wakefulness and depression during sleep. Furthermore, slope and amplitude of cortical evoked responses increase after wakefulness, decrease after sleep and correlate with changes in slow-wave activity, a marker of sleep pressure. Changes in molecular and electrophysiological indicators of synaptic strength are largely independent of the time of day. Finally, cortical long-term potentiation can be easily induced after sleep, but not after wakefulness. Thus, wakefulness appears to be associated with net synaptic potentiation, whereas sleep may favor global synaptic depression, thereby preserving an overall balance of synaptic strength.