Comparison of tears and lacrimal gland fluid in the rabbit and guinea pig.
Comparison of tears and lacrimal gland fluid in the rabbit and guinea pig.
复制标题
兔子和豚鼠眼泪和泪腺液的比较。
DOI：
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发表时间：
1985
影响因子：
2
通讯作者：
N. V. van Haeringen
中科院分区：
文献类型：
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作者：
L. Thörig;E. J. van Agtmaal;E. Glasius;K. L. Tan;N. V. van Haeringen
对家兔和豚鼠的泪腺排泄管进行原位插管，以收集纯净的泪腺液，不受哈氏腺分泌物或结膜和角膜上皮脱落细胞的污染。十二烷基硫酸钠-聚丙烯酰胺 (SDS-PAA) 梯度平板凝胶电泳后，两个物种的眼泪和泪腺液均显示出物种特异性和分子量依赖性模式。这两个物种最显着的差异是泪液中存在与血清白蛋白相对应的蛋白质，而泪腺液中几乎缺乏。同样，还测量了眼泪和泪腺液中的多种酶、总蛋白和 PGE2。对于兔泪液，泪腺是溶菌酶（LZM）、β-己糖胺酶（β-hex）、血管紧张素转换酶（ACE）、纤溶酶原激活剂（PA）和总蛋白的主要组织来源，而乳酸脱氢酶（LDH）和大部分前列腺素E2（PGE2）主要作为其他眼组织来源的产物存在于兔泪液中。在豚鼠眼泪中，泪腺激发过氧化物酶（POD）、ACE、PA和少量PGE2，淀粉酶（AMY）、LDH和大量PGE2通过其他眼组织来源添加到豚鼠眼泪中。 Beta-hex 和总蛋白从泪腺和其他眼组织来源释放。
The excretory duct of the lacrimal gland of rabbits and guinea pigs was cannulated in situ for collection of pure lacrimal gland fluid, not contaminated by secretions from the Harderian gland or contributions of desquamating cells of the conjunctival and corneal epithelium. Tears as well as lacrimal gland fluid of both species showed a species-specific and molecular weight-dependent pattern after sodium dodecylsulphate-polyacrylamide (SDS-PAA) gradient slab gel electrophoresis. The most striking difference in both species was a protein corresponding to serum albumin present in tears and almost lacking in lacrimal gland fluid. Likewise, a variety of enzymes, total protein and PGE2 were measured in tears and lacrimal gland fluid. For rabbit tears the lacrimal gland is the primary tissue source of lysozyme (LZM), beta-hexosaminidase (beta-hex), angiotensin-converting enzyme (ACE), plasminogen activator (PA) and total protein, while lactate dehydrogenase (LDH) and the greater part of prostaglandin E2 (PGE2) are present in rabbit tears mainly as products from other ocular tissue sources. In guinea pig tears peroxidase (POD), ACE, PA and less PGE2 are exceted by the lacrimal gland, amylase (AMY), LDH and a substantial amount of PGE2 are added to the guinea pig tears by other ocular tissue sources. Beta-hex and total protein are released from the lacrimal gland and from other ocular tissue sources as well.