Studies on immobilized papain
Studies on immobilized papain
复制标题
固定化木瓜蛋白酶的研究
DOI：
10.1002/bit.260150303
复制
发表时间：
1973
影响因子：
3.8
通讯作者：
R. Mason
中科院分区：
文献类型：
--
作者：
H. H. Weetall;R. Mason
木瓜蛋白酶通过几种不同的方法与ZrO2−涂层多孔玻璃共价偶联。对这些衍生物进行了表征，并利用酪蛋白底物测定了它们的半衰期。木瓜蛋白酶共价偶联到多孔玻璃上，之前通过酰胺键转化为羧酸衍生物，其半衰期为35天。
Papain was covalently coupled to ZrO2−coated porous glass by several different methods. These derivatives were characterized and their operational half‐lives determined using casein substrate. Papain covalently coupled to the porous glass, previously converted to a carboxylic acid derivative, through amide linkage gave a 35 day operational half‐life.