Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release.
Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release.
复制标题
DOI：
10.1111/2049-632x.12061
复制
发表时间：
2013-10
影响因子：
3.3
通讯作者：
Armstrong SK
中科院分区：
文献类型：
--
作者：
Hanawa T;Yonezawa H;Kawakami H;Kamiya S;Armstrong SK
百日咳博德特氏菌是人类疾病百日咳的细菌病原体。在许多细菌中，细胞外功能 sigma 因子 σE 是对包膜应激反应的核心，其活性受到 RseA 抗 sigma 因子的负向控制。在本研究中，研究了 RseA 在百日咳博德特氏菌包膜应激反应中的作用。与野生型菌株相比，rseA 突变体表现出更高的对包膜应激的抵抗力并在 25°C 下增强生长。 rpoH 和其他预测的 σE 靶基因证明，与野生型亲本相比，rseA 突变体的转录增加。在包膜胁迫下，野生型百日咳博德特氏菌和大肠杆菌中这些基因的转录也有所增加，而在 rseA 突变体中没有观察到胁迫诱导的转录增加。 rseA 失活还与培养物上清液中某些蛋白质水平的改变有关，这表明腺苷酸环化酶毒素 (CyaA) 水平增加。突变体中 CyaA 的增加与细胞外毒素稳定性的明显增加以及含有 CyaA 的外膜囊泡的产生增加相关。与此一致的是，与野生型菌株相比，rseA突变细胞产生数量增加的大表面相关囊泡。
Bordetella pertussis is the bacterial agent of the human disease, whooping cough. In many bacteria, the extracellular function sigma factor σE is central to the response to envelope stress, and its activity is negatively controlled by the RseA anti-sigma factor. In this study, the role of RseA in B. pertussis envelope stress responses was investigated. Compared with the wild-type strain, an rseA mutant showed elevated resistance to envelope stress and enhanced growth at 25°C. rpoH and other predicted σE target genes demonstrated increased transcription in the rseA mutant compared with the wild type parent. Transcription of those genes was also increased in wild type B. pertussis and Escherichia coli under envelope stress, whereas no stress-induced increase in transcription was observed in the rseA mutant. rseA inactivation was also associated with altered levels of certain proteins in culture supernatant fluids, which showed increased adenylate cyclase toxin (CyaA) levels. The increased CyaA in the mutant was correlated with an apparent increased stability of the extracellular toxin and increased production of CyaA-containing outer membrane vesicles. Consistent with this, compared with the wild type strain, rseA mutant cells produced increased numbers of large surface-associated vesicles.