Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana
Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana
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DOI：
10.1023/a:1023904217641
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发表时间：
2003-05-01
影响因子：
5.1
通讯作者：
Kohchi, T
中科院分区：
文献类型：
--
作者：
Hyodo, H;Yamakawa, S;Kohchi, T
细胞壁结构的调节在植物的生长和分化中起着核心作用。木葡聚糖内切葡糖苷酶/水解酶(XTHs)催化木聚糖链的断裂和分子接枝，在细胞壁的松动和重排中起作用。我们对XTH家族的一个成员XTH9进行了研究，该家族是通过系统差异筛选拟南芥茎尖高表达基因而被分离出来的。在营养生长期，XTH9转录本在顶端积累。在生殖生长期，顶端的转录水平进一步增加，在花蕾、花梗和结花的节间也检测到转录水平。XTH9的表达水平在以节间细胞长度较短为特征的acl等突变体中显著降低，但在温度敏感的acl突变体中在允许温度下恢复。XTH9在花序茎上的差异表达也在Pin1中检测到，在那里没有形成侧器官。这些观察表明，XTH9的表达与植物的发育是协调的，包括营养分生组织和生殖分生组织的分化以及花序茎的细胞伸长。
Regulation of cell wall structure plays a central role in growth and differentiation in plants. Xyloglucan endotransglucosylase/hydrolases (XTHs) that catalyze the cleavage and molecular grafting of xyloglucan chains function in loosening and rearrangement of the cell wall. We have characterized XTH9, a member of the XTH family that was isolated by systematic differential screening for highly expressed genes in shoot apices in Arabidopsis. In the vegetative phase, XTH9 transcripts accumulate in the shoot apex region. In the reproductive phase, transcript levels in shoot apices increase further, and they also are detected in flower buds, flower stalks and internodes bearing flowers. XTH9 expression levels were reduced markedly in mutants such as acl which are characterized by short internodal cell lengths, but recover at permissive temperatures in the temperature-sensitive acl mutants. Differential expression of XTH9 along the inflorescence stem was also detected in pin1 where no lateral organs are formed. These observations suggest that XTH9 expression is coordinated with plant development, including the differentiation from vegetative and reproductive meristems and cell elongation of the inflorescence stem.