Pump function of the human corneal endothelium. Effects of age and cornea guttata.
Pump function of the human corneal endothelium. Effects of age and cornea guttata.
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DOI：
10.1016/s0161-6420(85)33973-8
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发表时间：
1985-06
期刊：
影响因子：
13.7
通讯作者：
D. Geroski;Mamoru Matsuda;Richard W. Yee;H. Edelhauser
中科院分区：
文献类型：
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作者：
D. Geroski;Mamoru Matsuda;Richard W. Yee;H. Edelhauser
用氚哇巴因与内皮细胞Na/K-ATPase的特异性结合来定量测定人角膜内皮细胞内泵部位的密度。不适合用于角膜移植的供眼是从威斯康星狮子眼库获得的。用广域镜片显微镜检查每只供体眼的内皮细胞，并对镜片进行追踪和数字化，以确定细胞密度。哇巴因结合在配对的分离内皮片中被测量。共有26对供眼进行了研究，年龄从11岁到91岁不等。20对被确定为内皮正常的人被发现具有恒定的泵部位密度，该密度与供者年龄无关。有6对供体有中等的牙胶；在这组中，泵的部位密度显着增加。这些结果表明，尽管在人类角膜内皮中泵部位密度通常是恒定的，但增加内皮细胞通透性的条件，如滴眼液，可以导致泵部位密度和可能的泵功能的代偿性增加。
The specific binding of tritiated ouabain to endothelial Na/K ATPase was used to quantitate the density of pump sites in the human corneal endothelium. Donor eyes, unsuitable for use in keratoplasty, were obtained from the Wisconsin Lions Eye Bank. The endothelium of each donor eye was examined using wide-field specular microscopy, and the specular micrographs were traced and digitized for the determination of cell density. Ouabain binding was measured in matched pairs of isolated endothelial sheets. A total of 26 pairs of donor eyes, ranging in age from 11 through 91 years, were studied. Twenty pairs, determined to have normal endothelia, were found to have a constant pump site density which was independent of donor age. Six donor pairs had moderate guttata; in this group pump site density was significantly increased. These results indicate that, although pump site density is normally constant in the human corneal endothelium, conditions which increase endothelial permeability, such as guttata, can cause a compensatory increase in pump site density and presumably pump function.