Relationship between geographical origin, seed size and genetic diversity in faba bean (Vicia faba L.) as revealed by SSR markers
Relationship between geographical origin, seed size and genetic diversity in faba bean (Vicia faba L.) as revealed by SSR markers
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DOI：
10.1007/s00438-017-1326-0
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发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Frary, Anne
中科院分区：
文献类型：
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作者：
Gol, Aurhan;Doganlar, Sami;Frary, Anne
蚕豆（Vicia faba L.）是一种重要的豆类作物，因为其蛋白质和淀粉含量高。蚕豆能在不同的气候条件下生长，并且由于其根部的固氮菌，它是一种理想的轮作作物。在这项研究中，利用32对SSR引物对255份蚕豆种质资源进行了鉴定，共产生302个多态性片段。结果表明，根据邻接算法（r = 0.91），蚕豆个体被分为两个主要群体，同时也存在一些基于地理起源和种子大小的聚类。群体结构也得到了确定，并且与将这些种质资源分为两个亚群体的聚类分析结果一致。分子方差分析（AMOVA）显示群体内遗传变异水平较高。遗传相似性和地理邻近性与欧洲种质资源和非洲及亚洲种质资源的分离有关。有趣的是，地方品种（38%）和栽培品种（40%）的多样性之间没有显著差异，这表明由于育种，遗传变异性尚未丧失。总共选择了44个具有良好遗传特征的蚕豆个体作为核心种质，以便进一步对产量和营养性状进行检测。
Faba bean (Vicia faba L.) is an important legume species because of its high protein and starch content. Broad bean can be grown in different climatic conditions and is an ideal rotation crop because of the nitrogen fixing bacteria in its roots. In this work, 255 faba bean germplasm accessions were characterized using 32 SSR primers which yielded 302 polymorphic fragments. According to the results, faba bean individuals were divided into two main groups based on the neighbor-joining algorithm (r = 0.91) with some clustering based on geographical origin as well as seed size. Population structure was also determined and agreed with the dendrogram analysis in splitting the accessions into two subpopulations. Analysis of molecular variance (AMOVA) revealed high levels of within population genetic variation. Genetic similarity and geographical proximity were related with separation of European accessions from African and Asian ones. Interestingly, there was no significant difference between landrace (38%) and cultivar (40%) diversity indicating that genetic variability has not yet been lost due to breeding. A total of 44 genetically well-characterized faba bean individuals were selected for a core collection to be further examined for yield and nutritional traits.