Surface characterization of hydroxyapatite and related calcium phosphates by XPS and TOF-SIMS
Surface characterization of hydroxyapatite and related calcium phosphates by XPS and TOF-SIMS
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DOI：
10.1021/ac990812h
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发表时间：
2000-07-01
影响因子：
7.4
通讯作者：
Ratner, BD
中科院分区：
文献类型：
--
作者：
Lu, HB;Campbell, CT;Ratner, BD
用X射线光电子能谱(XPS)和飞行时间二次离子质谱仪(TOF-SIMS)研究了六种具有生物意义的磷酸钙(CAP)相(羟基磷灰石、二水二水磷酸钙、二水钙、一元钙、β三元钙、八钙)的表面。O(1s)摆动卫星的强度与磷酸盐的氧含量有关。结合Ca/P和O/CaXPS峰比，这一特征有助于识别未知样品表面存在的帽状相(S)，并确定其摩尔分数，如骨骼样品所证实的那样。碳酸盐杂质的贡献可以用其在279.9 eV的C(1s)峰来量化，并从O(1s)线形中减去以帮助识别。主成分分析(PCA)被成功地应用于这六个帽状相的TOF-SIMS谱分析。多变量分析可以帮助使用前两个PC来区分这些帽状相，这两个PC仅由几个关键离子的相对强度主导：PO3-、O-、Ca+、CaOH+、PO2-和OH-。
The surfaces of six biologically interesting calcium phosphate (CaP) phases (hydroxyapatite, dibasic calcium phosphate dihydrate, dibasic calcium phosphate, monobasic calcium phosphate, beta-tribasic calcium phosphate, octacalcium phosphate) have been examined by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS), The intensity of an O(1s) shake-up satellite correlates with the phosphate oxygen content. Together with the Ca/P and O/Ca XPS peak ratios, this feature helps provide identification of the CaP phase(s) present in the surface of unknown samples and establish their mole fractions, as proven with a bone sample. Contributions from carbonate impurities can be quantified using its C(1s) peak at 279.9 eV and subtracted from the O(1s) line shape to aid identification. Principal component analysis (PCA) has been applied successfully to analyze TOF-SIMS spectra of these six CaP phases. Multivariate analysis can help differentiate these CaP phases using the first two PCs, which are dominated by the relative intensities of only a few key ions: PO3-, O-, Ca+, CaOH+, PO2-, and OH-.