The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes.
The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes.
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DOI：
10.1021/jp202353c
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
Tobias Pflock;S. Oellerich;J. Southall;R. Cogdell;G. Matthias Ullmann;J. Köhler
中科院分区：
文献类型：
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作者：
Tobias Pflock;S. Oellerich;J. Southall;R. Cogdell;G. Matthias Ullmann;J. Köhler
我们采用皮秒时间尺度上的时间分辨光谱结合动态Monte Carlo模拟，研究了紫色光合细菌Rhodopyreneacidophila的捕光2（LH 2）复合物的光物理性质。的荧光瞬态的变化进行了研究，作为一个功能的激发注量，激发和样品制备条件的重复率。在这里，我们提出了在洗涤剂增溶的LH 2复合物上获得的结果，即，避免intercomplex相互作用，并表明，一个简单的四态模型是足以掌握实验观测定量，而不需要任何自由参数。这种方法使我们能够定量测量孤立的、不相互作用的LH 2复合物中的单重态-三重态湮灭率。
We have employed time-resolved spectroscopy on the picosecond time scale in combination with dynamic Monte Carlo simulations to investigate the photophysical properties of light-harvesting 2 (LH2) complexes from the purple photosynthetic bacterium Rhodopseudomonas acidophila. The variations of the fluorescence transients were studied as a function of the excitation fluence, the repetition rate of the excitation and the sample preparation conditions. Here we present the results obtained on detergent solubilized LH2 complexes, i.e., avoiding intercomplex interactions, and show that a simple four-state model is sufficient to grasp the experimental observations quantitatively without the need for any free parameters. This approach allows us to obtain a quantitative measure for the singlet-triplet annihilation rate in isolated, noninteracting LH2 complexes.