USE OF MUTATED FLP RECOGNITION TARGET (FRT) SITES FOR THE EXCHANGE OF EXPRESSION CASSETTES AT DEFINED CHROMOSOMAL LOCI
USE OF MUTATED FLP RECOGNITION TARGET (FRT) SITES FOR THE EXCHANGE OF EXPRESSION CASSETTES AT DEFINED CHROMOSOMAL LOCI
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DOI：
10.1021/bi00209a003
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发表时间：
1994-11-01
期刊：
影响因子：
2.9
通讯作者：
BODE, J
中科院分区：
文献类型：
--
作者：
SCHLAKE, T;BODE, J
使用的FLP/FRT系统的位点特异性重组和野生型识别位点（FRT）与某些突变FRT网站，它成为可能挑起，高产量，在培养的哺乳动物鳗鱼的双互惠交换事件。它表明，该技术使表达盒靶向受体细胞中的适当的染色体参考位点，以改善反向遗传学的概念。突变FRT网站的设计，促进这样一个过程将划定。我们的研究结果表明，测试的五个间隔区突变与野生型一样具有功能，但在交叉重组的程度上有所不同，必须最大限度地减少同时使用。
Using the FLP/FRT system for site-specific recombination and the wild-type recognition site (FRT) in conjunction with certain mutant FRT sites, it becomes possible to provoke, with high yield, a double-reciprocal crossover event in cultured mammalian eels. It is demonstrated that this technology enables a targeting of expression cassettes to appropriate chromosomal reference sites in the recipient cell to improve the concepts of reverse genetics. The design of mutant FRT sites promoting such a process will be delineated. Our results show that the five spacer mutations tested are functional as the wild type but differ in the extent of their cross-recombination, which has to be minimized for their simultaneous usage.