High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography
High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography
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DOI：
10.1364/boe.6.002713
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发表时间：
2015-07-01
影响因子：
3.4
通讯作者：
Larina, Irina V.
中科院分区：
文献类型：
--
作者：
Burton, Jason C.;Wang, Shang;Larina, Irina V.
对人类生殖事件和调节生育和不育的分子机制的理解在很大程度上依赖于对小鼠模型中相应表型的分析。虽然分子遗传学方法提供了显着的洞察这些过程的分子调控，缺乏现场成像方法，允许详细的可视化小鼠生殖器官限制了我们的调查动态事件发生在排卵，受精和胚胎发育的植入前阶段。在这里，我们介绍了一种在体内三维成像的方法，可视化的小鼠输卵管和生殖活动的微观尺度的空间分辨率，使用光学相干断层扫描（OCT）。该方法依赖于自然组织光学对比度，并且不需要应用任何造影剂。这是第一次，我们提供了输卵管内部结构特征的高分辨率图像，以及其他生殖器官和卵丘细胞包围的卵母细胞。这些结果为广泛的生活动态研究提供了基础，重点是了解生育力和不孕症。(C)2015美国光学学会
The understanding of the reproductive events and the molecular mechanisms regulating fertility and infertility in humans relies heavily on the analysis of the corresponding phenotypes in mouse models. While molecular genetic approaches provide significant insight into the molecular regulation of these processes, the lack of live imaging methods that allow for detailed visualization of the mouse reproductive organs limits our investigations of dynamic events taking place during the ovulation, the fertilization and the pre-implantation stages of embryonic development. Here we introduce an in vivo three-dimensional imaging approach for visualizing the mouse oviduct and reproductive events with micro-scale spatial resolution using optical coherence tomography (OCT). This method relies on the natural tissue optical contrast and does not require the application of any contrast agents. For the first time, we present live high-resolution images of the internal structural features of the oviduct, as well as other reproductive organs and the oocytes surrounded by cumulus cells. These results provide the basis for a wide range of live dynamic studies focused on understanding fertility and infertility. (C) 2015 Optical Society of America