Manipulation of Pluripotent Stem Cell Metabolism for Clinical Application.
Manipulation of Pluripotent Stem Cell Metabolism for Clinical Application.
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DOI：
10.1007/s40778-017-0073-9
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发表时间：
2017
影响因子：
1.4
通讯作者：
Fukuda K
中科院分区：
文献类型：
--
作者：
Tohyama S;Tanosaki S;Someya S;Fujita J;Fukuda K
多能干细胞（Pluripotent stem cells，PSC）具有分化为多种细胞的能力，是再生治疗和药物筛选的理想细胞来源。然而，要实现PSC的临床应用，必须以低成本稳定制备大量高质量的靶细胞。为了实现这一目标，需要在残余PSC的重编程、分化和消除方面进行重大改进。本文就PSC代谢及其应用的最新研究进展作一综述。最近的研究表明，与分化的细胞相比，PSC具有不同的代谢谱。PSC的代谢特征对于维持多能性、自我更新、分化能力和细胞存活是不可或缺的。与用于分化和消除残余PSC的常规方法相比，代谢方法显示出改进的简单性、可扩展性和更低的成本。因此，PSC代谢的操纵将导致新的技术，以提高其效率。
Pluripotent stem cells (PSCs) have the capacity to differentiate into various types of cells, and are promising cell sources for regenerative therapy and drug screening. However, to realize the clinical application of PSCs, a large number of highly qualified target cells must be stably prepared with low cost. To achieve this, great improvements in the reprogramming, differentiation, and elimination of residual PSCs will be necessary. In this review, we summarize the updated knowledge about metabolism in PSCs and its application. Recent studies have shown that PSCs have distinct metabolic profiles compared to differentiated cells. The metabolic profiles of PSCs are indispensable for the maintenance of pluripotency, self-renewal, differentiation capacity, and cell survival. Metabolic approaches show improved simplicity, scalability, and lower cost than conventional methods for differentiation and elimination of residual PSCs. Thus, manipulation of PSC metabolism will lead to new technologies to improve their efficiencies.