In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish.
In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish.
复制标题
体内成像揭示成年斑马鱼成熟的少突胶质细胞分裂
DOI：
10.1186/s13619-021-00079-3
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发表时间：
2021-06-02
期刊：
影响因子：
--
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Zou S;Hu B
成熟的少突胶质细胞（mOLs）是否参与髓鞘再生一直存在争议几十年。最近的研究表明，mOL通过产生新的鞘参与髓鞘再生。然而，mOLs是否可以通过不对称分裂产生新的少突胶质细胞尚未得到证实。与其他物种相比，斑马鱼是研究髓鞘再生的完美模型。在这项研究中，视神经挤压没有诱导局部mOL死亡。从olig 2：eGFP鱼到AB/WT鱼的视神经移植后，来自供体的olig 2+细胞在受体中定居并重新包裹轴突。在移植后3个月将这些重新包装的olig 2+细胞鉴定为mOL后，体内成像显示olig 2+细胞增殖。此外，还在视网膜内捕获了来自mOL的新olig 2+细胞分裂的体内成像。最后，在髓鞘再生计划完成后，精细的视觉功能得到了恢复。总之，我们的体内成像结果表明，新的olig 2+细胞出生的mOL通过不对称分裂在成年斑马鱼，这突出了mOL在哺乳动物中枢神经系统髓鞘再生的进展中的作用。
Whether mature oligodendrocytes (mOLs) participate in remyelination has been disputed for several decades. Recently, some studies have shown that mOLs participate in remyelination by producing new sheaths. However, whether mOLs can produce new oligodendrocytes by asymmetric division has not been proven. Zebrafish is a perfect model to research remyelination compared to other species. In this study, optic nerve crushing did not induce local mOLs death. After optic nerve transplantation from olig2:eGFP fish to AB/WT fish, olig2+cells from the donor settled and rewrapped axons in the recipient. After identifying these rewrapping olig2+cells as mOLs at 3 months posttransplantation, in vivo imaging showed that olig2+cells proliferated. Additionally, in vivo imaging of new olig2+cell division from mOLs was also captured within the retina. Finally, fine visual function was renewed after the remyelination program was completed. In conclusion, our in vivo imaging results showed that new olig2+cells were born from mOLs by asymmetric division in adult zebrafish, which highlights the role of mOLs in the progression of remyelination in the mammalian CNS.
影响因子：
6.1
作者：
Lahne M;Hyde DR
通讯作者：
Hyde DR