Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and Shaking rat Kawasaki
Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and Shaking rat Kawasaki
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DOI：
10.1093/cercor/bhm148
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发表时间：
2008-05-01
期刊：
影响因子：
3.7
通讯作者：
Rockland, Kathleen S.
中科院分区：
文献类型：
--
作者：
Ichinohe, Noritaka;Knight, Adrian;Rockland, Kathleen S.
大鼠颗粒压后皮质（GRS）是一个简化的皮质，具有明显的分层，在最上层，具有明显的模块性。丘脑和皮层的输入隔离层，并在层1共定位，分别与顶端树突束起源于层2或5中的神经元。为了进一步研究这种组织，我们转向了reelin缺陷的reeler小鼠和Shaking大鼠川崎。我们发现，在这些啮齿类动物的尼氏染色中明显的被破坏的层压，实际上是隔离的传入神经和树突的斑块基质镶嵌。斑块由丘脑皮质连接组成，通过囊泡谷氨酸转运蛋白2（VGluT 2）或AChE可视化。周围基质由皮质皮质末梢组成，通过VGluT 1或锌可视化。树突集中在基质或斑块中，这取决于它们是OCAM阳性（基质）还是阴性（斑块）。在野生型啮齿类动物和可能的突变体中，OCAM（+）结构起源于第5层神经元。通过对树突（在固定切片中用荧光黄填充）和OCAM免疫荧光的双重标记，我们确定了卷轴中的2个群体：树突分支优选（假定的第5层神经元）或避免（假定的颗粒上神经元）OCAM 1基质。我们的结论是，输入-目标的关系在很大程度上保留在突变体GRS和树突-树突相互作用，涉及OCAM影响的马赛克配置的形成。
The rat granular retrosplenial cortex (GRS) is a simplified cortex, with distinct stratification and, in the uppermost layers, distinct modularity. Thalamic and cortical inputs are segregated by layers and in layer 1 colocalize, respectively, with apical dendritic bundles originating from neurons in layers 2 or 5. To further investigate this organization, we turned to reelin-deficient reeler mouse and Shaking rat Kawasaki. We found that the disrupted lamination, evident in Nissl stains in these rodents, is in fact a patch-matrix mosaic of segregated afferents and dendrites. Patches consist of thalamocortical connections, visualized by vesicular glutamate transporter 2 (VGluT2) or AChE. The surrounding matrix consists of corticocortical terminations, visualized by VGluT1 or zinc. Dendrites concentrate in the matrix or patches, depending on whether they are OCAM positive (matrix) or negative (patches). In wild-type rodents and, presumably, mutants, OCAM(+) structures originate from layer 5 neurons. By double labeling for dendrites (filled by Lucifer yellow in fixed slice) and OCAM immunofluorescence, we ascertained 2 populations in reeler: dendritic branches either preferred (putative layer 5 neurons) or avoided (putative supra-granular neurons) the OCAM 1 matrix. We conclude that input-target relationships are largely preserved in the mutant GRS and that dendrite-dendrite interactions involving OCAM influence the formation of the mosaic configuration.