Covalent Framework Particles Modified with MnO2 Nanosheets and Au Nanoparticles as Electrochemical Immunosensors for Human Chorionic Gonadotropin
Covalent Framework Particles Modified with MnO2 Nanosheets and Au Nanoparticles as Electrochemical Immunosensors for Human Chorionic Gonadotropin
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MnO2 纳米片和 Au 纳米颗粒修饰的共价框架颗粒作为人绒毛膜促性腺激素的电化学免疫传感器
DOI：
10.1021/acsanm.1c00199
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发表时间：
2021-04-15
影响因子：
5.9
通讯作者：
Li, Can-Peng
中科院分区：
文献类型：
--
作者：
Liang, Huan;Ning, Guobao;Li, Can-Peng
采用绿色、简便的方法制备了共价有机骨架(COF)/二氧化锰(MnO2)纳米复合材料。以刻蚀的埃洛石纳米管(EHNT)为传感平台材料，以CoF/MnO2纳米复合材料为信号放大材料，构建了检测人绒毛膜促性腺激素(HCG)的电化学生物传感器。用修饰了eHNT的金纳米颗粒(Au NPs)固定一抗(AB1)。随后，制备了Au/COF/MnO2复合材料，用于固定TB的二次抗体(AB2)和丰富的氧化还原信号，形成AB2/Tb/Au/COF/MnO2生物结合物。此外，在H_2O_2存在下，由于eHNT和AB_2生物偶联物的催化能力增强了H_2O_2还原的电催化作用，进一步获得了放大的电化学信号。在最佳条件下，所构建的电化学传感器的线性范围为0.0005-100mIU mL(-1)。所研制的传感器用于血清中人绒毛膜促性腺激素的检测，灵敏度高、重复性好、特异性好。免疫传感器的放大电流是由于Au/EHNT、CoF/MnO2纳米载体和Au NPs电催化剂优异的电荷转移特性造成的。本工作不仅为制备CoF/MnO2纳米复合材料提供了一种简便的方法，而且为HCG的电化学检测提供了一种超灵敏的分析方法。
A covalent organic framework (COF)/manganese dioxide (MnO2) nanocomposite (COF/MnO2) was prepared via a green and facile method. Using an etched halloysite nanotube (eHNT) as a material on the sensing platform and the COF/MnO2 nanocomposite for signal amplification, an electrochemical biosensor was constructed for detecting human chorionic gonadotropin (HCG). Gold nanoparticles (Au NPs) decorated with eHNT were used to immobilize primary antibodies (Ab1). Subsequently, Au/COF/MnO2 composites were prepared for immobilizing secondary antibodies (Ab2) and abundant redox signals of TB to form the Ab2/TB/Au/COF/MnO2 bioconjugate. Furthermore, in the presence of H2O2, the amplified electrochemical signal was further obtained, since the catalytic ability of eHNT and Ab2 bioconjugate improved the electrocatalysis of H2O2 reduction. Under the optimum conditions, the constructed electrochemical sensor showed a wider linear range of 0.0005-100 mIU mL(-1). Our prepared sensor was used to measure HCG in serum with ultrasensitivity, good reproducibility, and excellent specificity. Amplified current of the immunosensor was caused by outstanding charge transfer properties of the Au/eHNT, COF/MnO2 nanocarrier, and Au NPs electrocatalyst. This work not only provided a facile method for preparation of the COF/MnO2 nanocomposite but also developed an ultrasensitive assay for the electrochemical detection of HCG.