Evaluation of a soybean lectin-based method for the enrichment of erythroblasts
Evaluation of a soybean lectin-based method for the enrichment of erythroblasts
复制标题
DOI：
10.1369/jhc.4b6408.2005
复制
发表时间：
2005-03-01
影响因子：
3.2
通讯作者：
Hahn, S
中科院分区：
文献类型：
--
作者：
Babochkina, T;Mergenthaler, S;Hahn, S
我们进行了一项比较研究的富集成红细胞的大豆凝集素半乳糖特异性凝集素的方法和标准化的磁性细胞分选（MACS）协议。从11名妊娠11至40周的孕妇中获得的血液样本被分开，并通过每种方法进行平行检查。通过凝集素方法回收的成红细胞数量比通过MACS获得的数量高约8倍。我们的数据表明，凝集素为基础的方法可以提供一个更好的方法富集罕见的胎儿母血成红细胞。
We performed a comparative study of the enrichment of erythroblasts by a soybean agglutinin galactose-specific lectin method and a standardized magnetic cell-sorting (MACS) protocol. Blood samples, obtained from 11 pregnant women at between 11 and 40 weeks of gestation, were split and examined by each method in parallel. The number of erythroblasts recovered by the lectin method was approximately eightfold higher than the number obtained by MACS. Our data suggest that the lectin-based method may provide a better approach for the enrichment of rare fetal erythroblasts from maternal blood.