Promotion of B cell immune responses via an alum-induced myeloid cell population
Promotion of B cell immune responses via an alum-induced myeloid cell population
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DOI：
10.1126/science.1089926
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发表时间：
2004-06-18
期刊：
影响因子：
56.9
通讯作者：
Cambier, JC
中科院分区：
文献类型：
--
作者：
Jordan, MB;Mills, DM;Cambier, JC
幼稚B细胞暴露于细胞因子白细胞介素-4（IL-4）和/或抗原导致“引发”状态，其中主要组织相容性复合物II类分子的随后聚集诱导钙离子的动员和细胞增殖。然而，目前尚不清楚这种引发对免疫应答的重要性，也不清楚它通常是如何在体内诱导的。用常用的佐剂明矾注射小鼠导致脾B细胞的引发和先前未知的产生IL-4的Gr 1（+）细胞群体在脾中的积累。这些细胞和IL-4都是抗原特异性B细胞的体内引发和扩增以及抗体的最佳生产所需的。这些研究揭示了一个以前未知的辅助骨髓细胞群体在体液免疫反应的产生中的关键作用。
Exposure of naive B cells to the cytokine interleukin-4 (IL-4) and/or antigen leads to a state of "priming," in which subsequent aggregation of major histocompatibility complex class II molecules induces the mobilization of calcium ions and cell proliferation. However, it is not clear how critical this priming is for immune responses or how it is normally induced in vivo. Injection of mice with the commonly used adjuvant alum led to priming of splenic B cells and to the accumulation in the spleen of a previously unknown population of IL-4-producing, Gr1(+) cells. These cells and IL-4 were both required for in vivo priming and expansion of antigen-specific B cells, as well as for optimal production of antibody. These studies reveal a key role for a previously unknown accessory myeloid cell population in the generation of humoral immune responses.