Encapsulation of glucose oxidase and an oxygen-quenched fluorophore in polyelectrolyte-coated calcium alginate microspheres as optical glucose sensor systems
Encapsulation of glucose oxidase and an oxygen-quenched fluorophore in polyelectrolyte-coated calcium alginate microspheres as optical glucose sensor systems
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DOI：
10.1016/j.bios.2004.08.020
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发表时间：
2005-07-15
影响因子：
12.6
通讯作者：
McShane, MJ
中科院分区：
文献类型：
--
作者：
Brown, JQ;Srivastava, R;McShane, MJ
目前正在研究涂有纳米多层膜（PEM）的微球作为植入式生物传感器的潜在用途，即所谓的“智能纹身”。“在这项工作中，这种方法用于葡萄糖传感器的可行性是通过将葡萄糖氧化酶封装在海藻酸钙微球中，然后将氧淬灭的钌化合物包埋在相同的微结构中来证明的。这些微器件的一个新特点是在微球表面形成多层纳米膜，用于稳定酶的截留和控制底物的扩散。共聚焦显微镜用于确认传感器化学的稳定封装。传感器的葡萄糖的阶跃变化的可逆响应进行了观察，初步的实验数据进行了比较，由计算模型产生的理论预测。这些发现证明了所述纳米工程方法用于生产功能性可植入葡萄糖传感器材料的前景。(c)2004 Elsevier B.V.保留所有权利。
Microspheres coated with polyelectrolyte multilayers (PEM's) are being investigated for potential use as implantable biosensors-so-called "smart tattoos." In this work, the feasibility of this approach for glucose sensors was demonstrated by glucose oxidase encapsulated within calcium alginate microspheres, followed by entrapment of an oxygen-quenched ruthenium compound in the same microstructure. A novel feature of these microdevices is the formation of multilayer nanofilms on the surface of the microspheres, used to stabilize enzyme entrapment and control substrate diffusion. Confocal microscopy was used to confirm the stable encapsulation of sensor chemistry. The reversible response of sensors to step changes in glucose was observed, and preliminary experimental data were compared to theoretical predictions produced by a computational model. These findings demonstrate the promise of the described nanoengineering approach for production of functional implantable glucose sensor materials. (c) 2004 Elsevier B.V. All rights reserved.