Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications
Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications
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DOI：
10.1021/acsbiomaterials.8b01352
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发表时间：
2020-01-01
影响因子：
5.8
通讯作者：
Al Moustafa, Ala-Eddin
中科院分区：
文献类型：
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作者：
Augustine, Robin;Hasan, Anwarul;Al Moustafa, Ala-Eddin
细胞增殖、细胞迁移和血管生成不足是慢性糖尿病伤口不愈合的主要原因。在伤口敷料中加入氧化铈纳米颗粒（nCeO（2））可能是促进血管生成和糖尿病伤口愈合的一种有前途的方法。在本文中，我们报告了一种新的nCeO（2）的发展，含有静电纺丝聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）膜的糖尿病伤口愈合的应用。进行体外细胞粘附研究、鸡胚血管生成测定和体内糖尿病伤口愈合研究，以评估所开发的膜的细胞增殖、血管生成和伤口愈合潜力。实验结果表明，含nCeO（2）的PHBV膜作为伤口敷料，能促进细胞增殖和细胞粘附。对于小于1%w/w的nCeO（2）含量，人乳腺上皮细胞（HMEC）平行于PHBV的单个纤维粘附。对于高于1%w/w的nCeO（2）含量，细胞开始变平并遍布纤维。卵内血管生成实验显示nCeO（2）掺入的PHBV膜能够促进血管形成。糖尿病大鼠体内伤口愈合研究证实了nCeO（2）掺入的PHBV膜的伤口愈合潜力。该研究表明，nCeO（2）掺入的PHBV膜具有很强的潜力，可用作伤口敷料，以促进细胞增殖和血管化，并促进糖尿病伤口的愈合。
Insufficient cell proliferation, cell migration, and angiogenesis are among the major causes for nonhealing of chronic diabetic wounds. Incorporation of cerium oxide nanoparticles (nCeO(2)) in wound dressings can be a promising approach to promote angiogenesis and healing of diabetic wounds. In this paper, we report the development of a novel nCeO(2) containing electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membrane for diabetic wound healing applications. In vitro cell adhesion studies, chicken embryo angiogenesis assay, and in vivo diabetic wound healing studies were performed to assess the cell proliferation, angiogenesis, and wound healing potential of the developed membranes. The experimental results showed that nCeO(2) containing PHBV membranes can promote cell proliferation and cell adhesion when used as wound dressings. For less than 1% w/w of nCeO(2) content, human mammary epithelial cells (HMEC) were adhered parallel to the individual fibers of PHBV. For higher than 1% w/w of nCeO(2) content, cells started to flatten and spread over the fibers. In ovo angiogenic assay showed the ability of nCeO(2) incorporated PHBV membranes to enhance blood vessel formation. In vivo wound healing study in diabetic rats confirmed the wound healing potential of nCeO(2) incorporated PHBV membranes. The study suggests that nCeO(2) incorporated PHBV membranes have strong potential to be used as wound dressings to enhance cell proliferation and vascularization and promote the healing of diabetic wounds.