Polymerization of diacetylene phospholipid bilayers on solid substrate: influence of the film deposition temperature.
Polymerization of diacetylene phospholipid bilayers on solid substrate: influence of the film deposition temperature.
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DOI：
10.1021/la701346x
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
K. Morigaki;H. Schönherr;T. Okazaki
中科院分区：
文献类型：
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作者：
K. Morigaki;H. Schönherr;T. Okazaki
固体基质上的微图案磷脂双层为各种应用（例如高通量药物筛选）提供了一个有吸引力的平台。我们之前开发了一种基于光聚合的方法，用于生成由聚合和流体脂质双层组成的微图案双层。含丁二炔的磷脂（DiynePC）的光刻光聚合可以轻松制造流体脂质膜的分隔阵列。在此，我们报告了一个对所制造的聚合物双层的均匀性和质量有显着影响的关键实验参数，即单体 DiynePC 单层在水面上形成并通过 Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) 技术转移到固体基质上的温度。使用荧光显微镜和原子力显微镜发现，如果在单层的三相点温度（约 20 摄氏度）以下制备 DiynePC 双层，则聚合双层是均匀的，此时发生从气态到液态凝聚态的直接转变。高于该温度制备的双层的裂纹状线缺陷数量显着增加。差异归因于单层中从水面转移到基底的域结构。结论是，域的大小，而不是每个域中的分子堆积，在缺陷的形成中发挥着关键作用。假设双层的自发曲率和面积变化会导致聚合时薄膜与基材不稳定和分离。我们目前的结果强调了控制域结构对于技术应用中所需的聚合双层的均匀性的重要性。
Micropatterned phospholipid bilayers on solid substrates offer an attractive platform for various applications, such as high throughput drug screening. We have previously developed a photopolymerization-based methodology for generating micropatterned bilayers composed of polymerized and fluid lipid bilayers. Lithographic photopolymerization of a diacetylene-containing phospholipid (DiynePC) allowed facile fabrication of compartmentalized arrays of fluid lipid membranes. Herein, we report on a key experimental parameter that significantly influences the homogeneity and quality of the fabricated polymeric bilayers, namely the temperature at which monolayers of monomeric DiynePC were formed on the water surface and transferred onto solid substrates by the Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) technique. Using fluorescence microscopy and atomic force microscopy, it was found that polymerized bilayers were homogeneous, if bilayers of DiynePC were prepared below the triple point temperature (ca. 20 degrees C) of the monolayer, where a direct transition from the gaseous state to the liquid condensed state occurred. Bilayers prepared above this temperature had a markedly increased number of crack-like line defects. The differences were attributed to the domain structures in the monolayer that were transferred from the water surface to the substrate. Domain size, rather than the molecular packing in each domain, was concluded to play a critical role in the formation of defects. The spontaneous curvature and area changes of bilayers were postulated to cause destabilization and detachment of the films from the substrate upon polymerization. Our present results highlight the importance of controlling the domain structures for the homogeneity of polymerized bilayers required in technological applications.