CTCF participates in DNA damage response via poly(ADP-ribosyl)ation.
CTCF participates in DNA damage response via poly(ADP-ribosyl)ation.
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DOI：
10.1038/srep43530
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发表时间：
2017-03-06
影响因子：
4.6
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Han D;Chen Q;Shi J;Zhang F;Yu X
ccctc结合因子（CTCF）通过结合DNA链来确定活性DNA和异染色质DNA的界限，在染色质结构的调控中起着重要作用。然而，CTCF在DNA损伤反应中的作用尚不明确。在这里，我们发现CTCF被迅速招募到DNA损伤的部位。锌指结构域和聚adp核糖（PAR）介导了快速募集。进一步分析表明，只有三个锌指基序是PAR识别所必需的。此外，ctcf缺陷细胞对基因毒性应激如电离辐射（IR）敏感。综上所述，这些结果表明CTCF通过poly（adp -核糖基化）参与DNA损伤反应。
CCCTC-binding factor (CTCF) plays an essential role in regulating the structure of chromatin by binding DNA strands for defining the boundary between active and heterochromatic DNA. However, the role of CTCF in DNA damage response remains elusive. Here, we show that CTCF is quickly recruited to the sites of DNA damage. The fast recruitment is mediated by the zinc finger domain and poly (ADP-ribose) (PAR). Further analyses show that only three zinc finger motifs are essential for PAR recognition. Moreover, CTCF-deficient cells are hypersensitive to genotoxic stress such as ionizing radiation (IR). Taken together, these results suggest that CTCF participate in DNA damage response via poly(ADP-ribosylation).