SUBSTANCE-P IN THE HUMAN-BRAIN
SUBSTANCE-P IN THE HUMAN-BRAIN
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DOI：
10.1016/0306-4522(86)90041-2
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发表时间：
1986-03-01
期刊：
影响因子：
3.3
通讯作者：
CUELLO, AC
中科院分区：
文献类型：
--
作者：
MAI, JK;STEPHENS, PH;CUELLO, AC
用免疫酶标法研究了几十年前Oscar和Cecile Vogt收集的大量石蜡包埋人脑切片以及较新的尸检材料中P物质免疫反应部位的分布。这些研究表明，P物质免疫反应性被保存在档案材料中，从而详细描述了免疫反应阳性细胞体、纤维网络和束在人脑中的定位。之前在实验动物和人身上所做的观察得到了证实和推广。此外，P物质免疫反应阳性细胞体见于大多数皮质区域，在结节区、纹状体、黑质(尤其是与血管的关系)以及与黑素细胞相关的P物质成分的分布上有新的特点。通过对多个平面的半连续切片的分析，尝试重建一些P物质通路。免疫细胞化学与图像分析相结合，能够对P物质免疫反应物质的不同浓度进行一些测量，并使其与确定的解剖标志或脑啡肽免疫反应部位密切相关。
The distribution of substance P immunoreactive sites was investigated by immunoenzymatic methods in a large series of paraffin embedded human brain sections from the collection assembled by Oscar and Cecile Vogt several decades ago, as well as from more recent post-mortem material. These studies demonstrated that substance P immunoreactivity was preserved in archival material permitting a detailed account of the localization of immunoreactive cell bodies, fibre networks and tracts in the human brain. Previous observations made on experimental animals and man were confirmed and extended. Additionally, substance P immunoreactive cell bodies were seen in most cortical areas and novel features were noted in the distribution of substance P-containing elements in the tuberal region, corpus striatum, substantia nigra (particularly in relationship to blood vessels) and in association with melanin-containing cells. Reconstruction of some substance P pathways was attempted by the analysis of semi-serial sections in more than one plane. Immunocytochemistry, in combination with image analysis, enabled some measurements of the differential concentrations of substance P immunoreactive material to be made and allowed a close correlation of this with defined anatomical landmarks or enkephalin immunoreactive sites.