Expression profiles of inflammatory and immune-related genes in Atlantic salmon (Salmo salar L.) at early time post vaccination
Expression profiles of inflammatory and immune-related genes in Atlantic salmon (Salmo salar L.) at early time post vaccination
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DOI：
10.1016/j.vaccine.2005.07.034
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发表时间：
2005-12-01
期刊：
影响因子：
5.5
通讯作者：
Evensen, O
中科院分区：
文献类型：
--
作者：
Haugland, O;Torgersen, J;Evensen, O
用油基疫苗接种大西洋鲑鱼PARR不可避免地会引起注射部位的炎症。虽然潜在的机制还没有得到很好的理解或详细的研究，但在这里，我们报告了表达水平的时程变化。通过实时RT-PCR检测1L-1β、Mx、两个β-2-微球蛋白变异体和MHC 11类β，在多组分油佐剂疫苗接种后前2至19天进行评估。对疫苗接种的高度多变的个体反应使得在差减分析之前选择高应答者是至关重要的。根据上述表达谱，高应答个体在接种疫苗后2.8天和19天。被选作消减分析。根据推测的功能对克隆进行聚类，表明在涉及炎症的基因发生之前，涉及新陈代谢和细胞信号的基因最初上调。被认为是炎症标志物的滞后伪造物在接种后48h以上，而在接种后8d发现它们构成了上调转录的主要部分。苍蝇第19天，免疫球蛋白和T细胞受体基因等免疫相关基因，占上调基因的比例比早先的时间点更高。(C)2005爱思唯尔有限公司。保留所有权利。
Vaccination of Atlantic salmon parr with oil-based vaccines will inevitably cause inflammation at the site of injection. albeit the underlying mechanisms are not very well understood or studied in any detail, Here, we report time-course changes In expression levels. assessed by real-time RT-PCR of 1L-1 beta, Mx, two beta-2-microglobulin variants and MHC class 11 beta, front 2 to 19 days post vaccination with a multi-component oil-adjuvanted vaccine. Highly variable individual responses to vaccination make selection of high responders essential prior to subtractive analysis. Based on the above mentioned expression profiles, high-responding individuals at 2.8 and 19 days post vaccination. were selected for subtractive analysis. Clustering of clones according to putative function, sugggest in initial up-regulation of genes involved in metabolism and cell signalling, before onset of genes involved in inflammation. The lag-time forgeries considered as inflammatory markers was more than 48 h, while they were found to constitute the major part of up-regulated transcripts by 8 days post vaccination. fly day 19, immune-related genes like immunoglobulin and T cell-receptor genes, comprised a higher proportion of the up-regulated genes than at earlier time points. (c) 2005 Elsevier Ltd. All rights reserved.